Lymphoid cell line identification and the detection of cross contamination
| Autor: | Betty R. Conner, Ronald Glaser, Michele A. Pellegrino, Soldano Ferrone |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Herpesvirus 4
Human Lymphoma Palatine Tonsil Receptors Antigen B-Cell Plant Science Human leukocyte antigen Biology Virus Cell Line Antigen HLA Antigens hemic and lymphatic diseases medicine Humans Typing Lymphocytes Antigens Viral Karyotype medicine.disease Virology Burkitt Lymphoma Cell culture Karyotyping biology.protein Antibody Cell Division Biotechnology |
| Zdroj: | In vitro. 16(5) |
| ISSN: | 0073-5655 |
| Popis: | Four lymphoid cell lines, presumably recent derivatives from non-neoplastic human tonsils, were identified in actuality as established Burkitt, lymphoma and lymphoblastoid cell lines (LCL) when tested by chromosome banding and typing for HLA antigens. Additionally, the morphology, growth characteristics, tumorigenicity, expression of Epstein-Barr virus antigens and surface membrane immunoglobulins (SmIg) of the four lymphoid cell lines confirmed the correct identifications. In determining the possiblity of cross contamination, the most reliable criteria for the identification of lymphoid cell lines were found to be HLA antigenic profile, karyotype, cellular morphology, and growth characteristics. |
| Databáze: | OpenAIRE |
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