Effects of medium components in a glycerol-based medium on vitamin K (menaquinone-7) production by Bacillus subtilis natto in biofilm reactors
| Autor: | Ali Demirci, Aydin Berenjian, Ehsan Mahdinia |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Glycerol Vitamin K Bioengineering Bacillus subtilis 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Bioreactors 010608 biotechnology Yeast extract Food science Response surface methodology biology Chemistry Biofilm Vitamin K 2 General Medicine biology.organism_classification Yeast Culture Media 030104 developmental biology Biofilms Fermentation Industrial and production engineering Biotechnology |
| Zdroj: | Bioprocess and biosystems engineering. 42(2) |
| ISSN: | 1615-7605 |
| Popis: | Menaquinone-7 (MK-7) as the most important form of Vitamin K has been reported to have miraculous benefits such as preventing cardiovascular diseases and osteoporosis along with antitumor effects. Therefore, there have been numerous studies in the past decades to improve MK-7 production via microbial fermentation. Unfortunately, both solid and liquid state fermentation strategies that are utilized for MK-7 production, face fundamental operational and scale-up issues as well as intense heat and mass transfer problems during fermentation. In this regard, biofilm reactors seem to be a practical solution to overcome these issues and enhance the production in agitated liquid fermentation. Therefore, this study was undertaken to utilize biofilm reactors in investigating and optimizing different media components in a glycerol-based medium. Using response surface methodology, the effects of glycerol, yeast extract, and soytone were studied in the fermentation medium on MK-7 production in biofilm reactor. With a composition of 48.2 g/L of glycerol, 8.1 g/L of yeast extracts, 13.6 g/L of soytone and 0.06 g/L of K2HPO4, MK-7 concentrations could reach 14.7 ± 1.4 mg/L in biofilm reactors, which was 57% higher compared to the MK-7 concentration achieved in suspended-cell reactors under similar conditions, while glycerol was depleted by the end of the fifth day in biofilm reactors, but glycerol was never depleted in suspended-cell reactors. Evidently, biofilm reactors present a reliable strategy to address the operational issues that occur during MK-7 biosynthesis on an industrial scale production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink