The cellular site of virus replication in the intestine of chicks infected with an avian adenovirus
| Autor: | D. I. Clemmer, H. Ichinose |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Avian adenovirus
Adenoviridae Infections Fluorescent Antibody Technique Virus Replication Epithelium Lymphoid hyperplasia Inclusion Bodies Viral Ileum Virology medicine Terminal ileum Animals Cell Nucleus Microscopy Goblet cell biology Strain (chemistry) General Medicine biology.organism_classification medicine.anatomical_structure Microscopy Fluorescence Viral replication biology.protein medicine.symptom Antibody Chickens |
| Zdroj: | Archiv für die gesamte Virusforschung. 25:277-287 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01556556 |
| Popis: | The terminal ileum from 10 chicks infected orally with strain 93 avian adenovirus and 5 uninfected chicks were examined by the fluorescent antibody technique and conventional histologic methods. In the infected birds, cells containing viral antigen were scattered, relatively few in number, and present only in the epithelial layer forming the surface of the villi. Both columnar and goblet cells were infected. Neither clusters of infected cells nor contiguous infected cells were observed. On stained sections examined with the light microscope, hypertrophied epithelial cells containing nuclear inclusions were seen to have essentially the same sparse and scattered distribution as cells containing viral antigen as shown by fluorescence techniques. The overall architecture of the epithelium was normal and no lymphoid hyperplasia or inflammatory reaction was present. The fluorescent antibody localization and inclusions implicate the nuclei of epithelial cells as the site of viral replication in the intestine with the particular model employed. |
| Databáze: | OpenAIRE |
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