Plasma Lipidome Analysis by Liquid Chromatography-High Resolution Mass Spectrometry and Ion Mobility of Hypertriglyceridemic Patients on Extended-Release Nicotinic Acid: a Pilot Study

Autor: Michel Krempf, Khadija Ouguerram, Véronique Ferchaud-Roucher, Mickael Croyal, Thomas Moyon, Yassine Zair
Přispěvatelé: Physiologie des Adaptations Nutritionnelles [UMR_A1280] (PhAN), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), Centre Hospitalier Universiatire Hôtel-Dieu de Nantes (CHU Hôtel-Dieu), West Human Nutrition Research Center, Centre Hospitalier Universitaire de l'Hôtel Dieu (CHU Hôtel Dieu), Biogenouest CORSAIRE core facility, SFR F. Bonamy [UMS 016], Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes (UN)-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Very low-density lipoprotein
[SDV]Life Sciences [q-bio]
Pilot Projects
01 natural sciences
High-performance liquid chromatography
Mass Spectrometry
chemistry.chemical_compound
High-density lipoprotein
Pharmacology (medical)
Hypertriglyceridemia
Cross-Over Studies
General Medicine
Lipidome
Middle Aged
Lipids
3. Good health
Lipoproteins
LDL
Low-density lipoprotein
UPLC-HRMS
Cholesteryl ester
lipids (amino acids
peptides
and proteins)
Cardiology and Cardiovascular Medicine
Sphingomyelin
Adult
Lipoproteins
Hyperlipidemias
Niacin
03 medical and health sciences
Double-Blind Method
medicine
Humans
Triglycerides
Ionmobility spectrometry
Pharmacology
Chemical structural elucidation
Chromatography
business.industry
Phosphatidylethanolamines
010401 analytical chemistry
Lipidomic
Cholesterol
HDL
medicine.disease
0104 chemical sciences
030104 developmental biology
chemistry
Delayed-Action Preparations
business
Chromatography
Liquid
Zdroj: Cardiovascular Drugs and Therapy
Cardiovascular Drugs and Therapy, Springer Verlag, 2017, 31 (3), pp.269-279. ⟨10.1007/s10557-017-6737-y⟩
ISSN: 1573-7241
0920-3206
Popis: Background Plasma high triacylglycerols and low HDL-C concentration are associated with increased cardiovascular events. Extended-release nicotinic acid (ERN) was shown to reduce plasma triacylglycerols and total cholesterol but also to markedly increase high-density lipoprotein-cholesterol (HDL-C). No data on the effect of ERN on different species of triacylglycerol, cholesteryl ester, and phospholipids are available. In this study, we applied a nontargeted lipidomic approach to investigate the plasma and lipoproteins lipids profile of hypertriglyceridemic patients treated with ERN or a placebo in order to identify new lipids markers associated with this treatment. Methods Eight hypertriglyceridemic patients enrolled in a crossover randomized trial with ERN for 8 weeks and treated with 2 g/day of ERN or a placebo. Ultra-performance liquid chromatography (UPLC) coupled to high-resolution mass spectrometry (HRMS) was used in mass spectrometry energy mode (HRMSE) combined with ion mobility spectrometry to characterize the plasma and very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoprotein (HDL) lipidome. The accuracy and precision of the method were validated on plasma samples. Results Compared to placebo, among 155 plasma lipids characterized using UPLC-ESI-HRMS, a multivariate analysis revealed a significant increase of lysophosphatidylcholine (LPC 20:5), a significant decrease of phosphatidylethanolamine (PE 16:0/22:3) and sphingomyelin (SM d18:1/22:0) and a decrease of triacylglycerol (TG 16:0/16:1/18:2) after ERN treatment. Analysis of these lipids in lipoproteins showed an increase of LPC (20:5) in HDL, a decrease of PE (16:0/22: 3) in HDL and LDL, of SM (d18:1/22:0) in VLDL and LDL and of TG (16:0/16:1/18:2) in VLDL. Conclusion This lipidomic strategy characterized new specific lipid markers likely to be involved in the effect of ERN on cardiovascular risk opening a new strategy to analyze randomized controlled with this treatment.
Databáze: OpenAIRE
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