Compromised fidelity of endocytic synaptic vesicle protein sorting in the absence of stonin 2
| Autor: | M. Kasim Diril, Michael Kintscher, York Winter, Dietmar Schmitz, Martin Wienisch, Jörg Breustedt, Dmytro Puchkov, Seong Joo Koo, Tanja Maritzen, Jürgen Klingauf, Gerit Pfuhl, Volker Haucke, Natalia L. Kononenko |
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| Rok vydání: | 2013 |
| Předmět: |
Endosome
Vesicle-Associated Membrane Protein 2 Endocytic cycle Synaptophysin Nerve Tissue Proteins Endosomes Biology Endocytosis Synaptic vesicle Synaptic Transmission Synaptotagmin 1 Mice Microscopy Electron Transmission Animals Mice Knockout Multidisciplinary Neuronal Plasticity Synaptotagmin I Brain Transport protein Cell biology Mice Inbred C57BL Adaptor Proteins Vesicular Transport Protein Transport PNAS Plus mossy fibers pHluorin hippocampus Synaptic Vesicles |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, 110(6): E526–E535 |
| ISSN: | 1091-6490 |
| Popis: | Neurotransmission depends on the exocytic fusion of synaptic vesicles (SVs) and their subsequent reformation either by clathrin-mediated endocytosis or budding from bulk endosomes. How synapses are able to rapidly recycle SVs to maintain SV pool size, yet preserve their compositional identity, is poorly understood. We demonstrate that deletion of the endocytic adaptor stonin 2 (Stn2) in mice compromises the fidelity of SV protein sorting, whereas the apparent speed of SV retrieval is increased. Loss of Stn2 leads to selective missorting of synaptotagmin 1 to the neuronal surface, an elevated SV pool size, and accelerated SV protein endocytosis. The latter phenotype is mimicked by overexpression of endocytosis-defective variants of synaptotagmin 1. Increased speed of SV protein retrieval in the absence of Stn2 correlates with an up-regulation of SV reformation from bulk endosomes. Our results are consistent with a model whereby Stn2 is required to preserve SV protein composition but is dispensable for maintaining the speed of SV recycling. |
| Databáze: | OpenAIRE |
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