Multilocus phylogenetic analysis of the genus Aeromonas
Autor: | Remedios Oncina, Arturo Monera, Monserrate Lopez-Alvarez, M. José Saavedra, Antonio J. Martínez-Murcia, Erica Lara, M. José Figueras |
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Rok vydání: | 2010 |
Předmět: |
Systematics
DNA Bacterial Sequence analysis Molecular Sequence Data Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Phylogenetics dnaX Ecology Evolution Behavior and Systematics Phylogeny 030304 developmental biology Genetics 0303 health sciences biology Phylogenetic tree Base Sequence 030306 microbiology Sequence Analysis DNA biology.organism_classification Housekeeping gene Bacterial Typing Techniques Aeromonas Evolutionary biology Genes Bacterial Taxonomy (biology) Sequence Alignment Multilocus Sequence Typing |
Zdroj: | Systematic and applied microbiology. 34(3) |
ISSN: | 1618-0984 |
Popis: | A broad multilocus phylogenetic analysis (MLPA) of the representative diversity of a genus offers the opportunity to incorporate concatenated inter-species phylogenies into bacterial systematics. Recent analyses based on single housekeeping genes have provided coherent phylogenies of Aeromonas. However, to date, a multi-gene phylogenetic analysis has never been tackled. In the present study, the intra- and inter-species phylogenetic relationships of 115 strains representing all Aeromonas species described to date were investigated by MLPA. The study included the independent analysis of seven single gene fragments (gyrB, rpoD, recA, dnaJ, gyrA, dnaX, and atpD), and the tree resulting from the concatenated 4705 bp sequence. The phylogenies obtained were consistent with each other, and clustering agreed with the Aeromonas taxonomy recognized to date. The highest clustering robustness was found for the concatenated tree (i.e. all Aeromonas species split into 100% bootstrap clusters). Both possible chronometric distortions and poor resolution encountered when using single-gene analysis were buffered in the concatenated MLPA tree. However, reliable phylogenetic species delineation required an MLPA including several "bona fide" strains representing all described species. |
Databáze: | OpenAIRE |
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