Leptospira interrogans serovar copenhageni harbors two lexA genes involved in SOS response
| Autor: | Josefa B. da Silva, Juliana S. Milanez, Luciane S. Fonseca, Zenaide Maria de Morais, Marilis V. Marques, Leonardo Hiroyuki Santos Momo, Silvio Arruda Vasconcellos, Claudia Barros Monteiro-Vitorello, Renata M. A. da Costa, Paulo Lee Ho |
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| Rok vydání: | 2012 |
| Předmět: |
DNA Repair
DNA repair Ultraviolet Rays Molecular Sequence Data Gene Dosage lcsh:Medicine Biology SOS box SOS Response (Genetics) Open Reading Frames Bacterial Proteins Gene Order Amino Acid Sequence SOS response Nucleotide Motifs lcsh:Science Promoter Regions Genetic SOS Response Genetics Gene Phylogeny Genetics Multidisciplinary Binding Sites lcsh:R Serine Endopeptidases MICROBIOLOGIA Gene Expression Regulation Bacterial biology.organism_classification Regulon Phenotype bacteria lcsh:Q Repressor lexA Leptospira interrogans Sequence Alignment Genome Bacterial Protein Binding Research Article |
| Zdroj: | PLoS ONE Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP PLoS ONE, Vol 8, Iss 10, p e76419 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Bacteria activate a regulatory network in response to the challenges imposed by DNA damage to genetic material, known as the SOS response. This system is regulated by the RecA recombinase and by the transcriptional repressor lexA. Leptospira interrogans is a pathogen capable of surviving in the environment for weeks, being exposed to a great variety of stress agents and yet retaining its ability to infect the host. This study aims to investigate the behavior of L. interrogans serovar Copenhageni after the stress induced by DNA damage. We show that L. interrogans serovar Copenhageni genome contains two genes encoding putative LexA proteins (lexA1 and lexA2) one of them being potentially acquired by lateral gene transfer. Both genes are induced after DNA damage, but the steady state levels of both LexA proteins drop, probably due to auto-proteolytic activity triggered in this condition. In addition, seven other genes were up-regulated following UV-C irradiation, recA, recN, dinP, and four genes encoding hypothetical proteins. This set of genes is potentially regulated by LexA1, as it showed binding to their promoter regions. All these regions contain degenerated sequences in relation to the previously described SOS box, TTTGN 5CAAA. On the other hand, LexA2 was able to bind to the palindrome TTGTAN 10TACAA, found in its own promoter region, but not in the others. Therefore, the L. interrogans serovar Copenhageni SOS regulon may be even more complex, as a result of LexA1 and LexA2 binding to divergent motifs. New possibilities for DNA damage response in Leptospira are expected, with potential influence in other biological responses such as virulence. |
| Databáze: | OpenAIRE |
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