In vitro antiplasmodial activity of callus culture extracts and fractions from fresh apical stems of **Phyllanthus niruri** L. (Euphorbiaceae): part 2

Autor: Arnold J. Vlietinck, Sandra Apers, K. Mesia, Nina Hermans, N. Luyindula, Luc Pieters, T. De Bruyne, Mariano Lusakibanza, S. Van Miert, J. Totté, L. Tona, R.K. Cimanga, C.T. Musuamba
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Zdroj: Journal of ethnopharmacology
ISSN: 0378-8741
Popis: The ethanolic extracts from fresh apical stems of Phyllanthus niruri L. (Euphorbiaceae) cultured on Murashige and Skoog (MS) medium supplemented with IBA/BAP/Coco nucifera L. milk for 1, 2, 4 and 6 months were phytochemically and biologically investigated and compared with intact plant part and whole plant extracts. Results from the in vitro antiplasmodial testing indicated that the EtOH extract of a 1-month-old callus culture (IC(50) = 16.3 +/- 2.5 microg/ml) exhibited a higher activity than the ethanolic extracts of the fresh apical stem (IC(50) = 18.2 +/- 2.4 microg/ml) and callus cultures of 2-, 4- and 6-months-old (25 microg/mlIC(50)40 microg/ml). These activities were however lower than that displayed by the ethanolic extract of the whole plant (IC(50)3 microg/ml). The EtOH extract of 1-month-old callus culture (the most active) was fractionated with solvents of different polarities. Its CH(2)Cl(2) fraction rich in terpenic constituents (IC(50) = 9.2 +/- 3.4 microg/ml) exhibited a higher antiplasmodial activity than its isoamylic alcohol fraction obtained at pH 2-3 (IC(50) = 25.6 +/- 2.3 microg/ml) rich in flavonoids. The activity of these two fractions was lower than that displayed by the same fractions from the whole plant (2 microg/mlIC(50)3 microg/ml). Alkaloidic fractions from the whole plant and 1-month-old callus culture of fresh apical stem were considered as inactive (IC(50)100 microg/ml).
Databáze: OpenAIRE
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