Protein Farnesyltransferase in Plants (Molecular Cloning and Expression of a Homolog of the [beta] Subunit from the Garden Pea)
| Autor: | C. L. Cramer, J. C. Watson, Zhenbiao Yang |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
DNA
Complementary Light Physiology Protein subunit Farnesyltransferase Molecular Sequence Data Plant Science Molecular cloning Genes Plant Polymerase Chain Reaction Transferases Complementary DNA Genetics Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Plant Proteins chemistry.chemical_classification Alkyl and Aryl Transferases Genome Plants Medicinal Base Sequence Sequence Homology Amino Acid biology cDNA library Nucleic Acid Hybridization Fabaceae Sequence Analysis DNA Molecular biology Amino acid Open reading frame Gene Expression Regulation chemistry Biochemistry biology.protein Research Article |
| Zdroj: | Plant Physiology. 101:667-674 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.101.2.667 |
| Popis: | Protein farnesyltransferase is a heterodimeric enzyme that attaches a farnesyl moiety to C-terminal cysteine residues. Both the alpha and beta subunits have recently been cloned and sequenced from yeast and rat. Degenerate oligonucleotides, corresponding to conserved regions of the beta subunit, were used as primers for the polymerase chain reaction to amplify cDNA synthesized from total cellular RNA from the apical buds of pea (Pisum sativum L.) seedlings. The 171-bp fragment obtained encodes an open reading frame of 57 amino acids showing 65% identity to the rat protein farnesyltransferase beta subunit. Using this fragment to screen a pea cDNA library, one full-length cDNA clone, designated PsFTb, was obtained that contains an open reading frame encoding a polypeptide of 419 amino acids. The predicted amino acid sequence exhibits 48 and 40% identity to the rat and yeast beta subunits, respectively, indicating that this cDNA encodes a pea homolog of the beta subunit of farnesyltransferase. Gel blot hybridizations show that PsFTb is likely to be encoded by a single-copy gene and is expressed as a transcript of approximately 1.7 kb. During photoregulated leaf development in continuous white light, PsFTb transcript levels within apical buds decline by approximately 5-fold. |
| Databáze: | OpenAIRE |
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