Additional file 1 of A novel and atypical NF-KB pro-inflammatory program regulated by a CamKII-proteasome axis is involved in the early activation of Muller glia by high glucose

Autor: Sbardella, Diego, Tundo, Grazia Raffaella, Mecchia, Alice, Palumbo, Camilla, Atzori, Maria Grazia, Levati, Lauretta, Boccaccini, Alessandra, Caccuri, Anna Maria, Cascio, Paolo, Lacal, Pedro Miguel, Graziani, Grazia, Varano, Monica, Coletta, Massimiliano, Parravano, Mariacristina
Rok vydání: 2022
DOI: 10.6084/m9.figshare.20329681.v1
Popis: Additional file 1: Fig. S1. Effect of high glucose treatment on IkBα expression over 60 min. Figure S2: DMSO, KT5720 and Inh. XII treatment does not significantly alter proteasome activity and composition under resting conditions. Figure S3: Silencing of CaMKIIα expression induces a drop of pRpt6(ser120)/Rpt6 ratio. Figure 4: DMSO, KT5720 and Inh. XII treatment does not modulate NF-kB signalling pathway under resting conditions. Figure S5: Generation of rMC1 clones expressing the Rpt6_S120A phosphodead mutant showing enhanced proteasome activity. Figure S6: Rpt6_S120A clones show constitutively elevate NF-kB activation. Figure S7: Determination of the dynamic linear range of actin and Ponceau.
Databáze: OpenAIRE