Cefquinome-loaded microsphere formulations against Klebsiella pneumonia infection during experimental infections

Autor: Cunchun Dai, Zhihui Hao, Li Zhao, Yuwen Li, Jiajia Zhu, Shaoqi Qu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Time Factors
Interleukin-1beta
Pharmaceutical Science
Cefquinome
02 engineering and technology
Two-fluid nozzle spray drier
Pharmacology
polylactic acid microspheres
chemistry.chemical_compound
Polylactic acid
Lung
media_common
Drug Carriers
Chemistry
General Medicine
021001 nanoscience & nanotechnology
Microspheres
Anti-Bacterial Agents
Klebsiella pneumoniae
Drug delivery
Host-Pathogen Interactions
Inflammation Mediators
0210 nano-technology
medicine.drug
Research Article
cefquinome
Drug
Surface Properties
media_common.quotation_subject
Drug Compounding
Polyesters
030106 microbiology
03 medical and health sciences
In vivo
medicine
pharmacodynamics
Pneumonia
Bacterial
Animals
Technology
Pharmaceutical
Particle Size
Rats
Wistar
Klebsiella pneumonia
Tumor Necrosis Factor-alpha
lcsh:RM1-950
Interleukin-8
medicine.disease
In vitro
Bacterial Load
Cephalosporins
Klebsiella Infections
Disease Models
Animal
Drug Liberation
lcsh:Therapeutics. Pharmacology
Pharmacodynamics
Zdroj: Drug Delivery
Drug Delivery, Vol 25, Iss 1, Pp 909-915 (2018)
ISSN: 1521-0464
1071-7544
Popis: The aim of this study was to prepare cefquinome-loaded polylactic acid microspheres and to evaluate their in vitro and in vivo characteristics and pharmacodynamics for the therapy of pneumonia in a rat model. Microspheres were prepared using a 0.7 mm two-fluid nozzle spray drier in one step resulting in spherical and smooth microspheres of uniform size (9.8 ± 3.6 μm). The encapsulation efficiency and drug loading of cefquinome were 91.6 ± 2.6% and 18.7 ± 1.2%, respectively. In vitro release of cefquinome from the microspheres was sustained for 36 h. Cefquinome-loaded polylactic acid microspheres as a drug delivery system was successful for clearing experimental Klebsiella pneumonia lung infections. A decrease in inflammatory cells and an inhibition of inflammatory cytokines TNF-α, IL-1β and IL-8 after microspheres treatment was found. Changes in cytokine levels and types are secondary manifestations of drug bactericidal effects. Rats were considered to be microbiologically cured because the bacterial load was less than 100 CFU/g. These results also indicated that the spray-drying method of loading therapeutic drug into polylactic acid microspheres is a straightforward and safe method for lung-targeting therapy in animals.
Databáze: OpenAIRE
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