Chemical structure and in vitro antitumor activity of rhamnolipids from Pseudomonas aeruginosa BN10
| Autor: | Ivanka Stoineva, Boryana Tuleva, Spiro Konstantinov, Anton Kril, Biliana Nikolova, Milka Georgieva, Neli Christova, Ivan Terziyski |
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| Rok vydání: | 2011 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization BALB 3T3 Cells Magnetic Resonance Spectroscopy Cell Survival Chemical structure Bioengineering Antineoplastic Agents Biology medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Microbiology Mice Surface-Active Agents Glycolipid Cell Line Tumor medicine Cytotoxic T cell Potency Animals Humans Lymphocytes Molecular Biology Pseudomonas aeruginosa Pseudomonas General Medicine biology.organism_classification In vitro Cell culture Glycolipids Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 170(3) |
| ISSN: | 1559-0291 |
| Popis: | A newly isolated indigenous strain BN10 identified as Pseudomonas aeruginosa was found to produce glycolipid (i.e., rhamnolipid-type) biosurfactants. Two representative rhamnolipidic fractions, RL-1 and RL-2, were separated on silica gel columns and their chemical structure was elucidated by a combination of nuclear magnetic resonance and mass spectroscopy. Subsequently, their cytotoxic effect on cancer cell lines HL-60, BV-173, SKW-3, and JMSU-1 was investigated. RL-1 was superior in terms of potency, causing 50 % inhibition of cellular viability at lower concentrations, as compared to RL-2. Furthermore, the results from fluorescent staining analysis demonstrated that RL-1 inhibited proliferation of BV-173 pre-B human leukemia cells by induction of apoptotic cell death. These findings suggest that RL-1 could be of potential for application in biomedicine as a new and promising therapeutic agent. |
| Databáze: | OpenAIRE |
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