T-Cell Phenotypes, Apoptosis and Inflammation in HIV+ Patients on Virologically Effective cART with Early Atherosclerosis
| Autor: | Kety Luzi, Alessandra Barassi, Antonella d'Arminio Monforte, Javier Sánchez Martínez, Elisa Suardi, Francesca Bai, Esther Merlini, Giulia Marchetti, Maddalena Cerrone, Gian Vico Melzi d’Eril |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
T-Lymphocytes lcsh:Medicine Apoptosis HIV Infections Cardiovascular Lymphocyte Activation Cohort Studies Molecular Cell Biology Medicine lcsh:Science Ultrasonography Multidisciplinary biology Cell Death virus diseases Middle Aged medicine.anatomical_structure Carotid Arteries Phenotype Anti-Retroviral Agents Cohort cardiovascular system Infectious diseases HIV clinical manifestations Female medicine.symptom Cohort study Research Article Cart Adult Combination therapy General Science & Technology T cell Immune Cells Inflammation Immunopathology Viral diseases Pharmacotherapy Antigens CD mental disorders Humans cardiovascular diseases Interleukin 6 Biology Aged business.industry lcsh:R Immunity HIV Atherosclerosis Immunology biology.protein lcsh:Q Clinical Immunology business |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 9, p e46073 (2012) |
| ISSN: | 1932-6203 |
| Popis: | OBJECTIVE: We investigated the potential relationship between T-cell phenotype, inflammation, endotoxemia, and atherosclerosis evaluated by carotid intima-media thickness (IMT) in a cohort of HIV-positive patients undergoing long-term virologically suppressive combination antiretroviral therapy (cART). DESIGN: We studied 163 patients receiving virologically suppressive cART. METHODS: We measured IMT (carotid ultrasound); CD4+/CD8+ T-cell activation (CD38, CD45R0), differentiation (CD127), apoptosis (CD95), and senescence (CD28, CD57) (flow cytometry); plasma sCD14, IL-6, TNF- α, sVCAM-1, hs-CRP, anti-CMV IgG (ELISA); LPS (LAL). The results were compared by Mann-Whitney, Kruskal-Wallis or Chi-square tests, and factors associated with IMT were evaluated by multivariable logistic regression. RESULTS: Of 163 patients, 112 demonstrated normal IMT (nIMT), whereas 51 (31.3%) had pathological IMT (pIMT: ≥1 mm). Of the patients with pIMT, 22 demonstrated an increased IMT (iIMT), and 29 were shown to have plaques. These patient groups had comparable nadir and current CD4+, VLs and total length of time on cART. Despite similar proportions of CD38-expressing CD8+ cells (p = .95), pIMT patients exhibited higher activated memory CD8+CD38+CD45R0+ cells (p = .038) and apoptotic CD4+CD95+ (p = .01) and CD8+CD95+ cells (p = .003). In comparison to nIMT patients, iIMT patients tended to have lower numbers of early differentiated CD28+CD57- memory CD4+ (p = .048) and CD28-CD57-CD8+ cells (p = .006), both of which are associated with a higher proliferative potential. Despite no differences in plasma LPS levels, pIMT patients showed significantly higher circulating levels of sCD14 than did nIMT patients (p = .046). No differences in anti-CMV IgG was shown. Although circulating levels of sCD14 seemed to be associated with a risk of ATS in an unadjusted analysis, this effect was lost after adjusting for classical cardiovascular predictors. CONCLUSIONS: Despite the provision of full viral suppression by cART, a hyperactivated, pro-apoptotic T-cell profile characterizes HIV-infected patients with early vascular damage, for whom the potential contribution of subclinical endotoxemia and anti-CMV immunity should be investigated further. |
| Databáze: | OpenAIRE |
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