Gonadal macrophage infiltration in congenital lipoid adrenal hyperplasia
| Autor: | Masanori Adachi, Tomonobu Hasegawa, Kenji Ihara, Tomohiro Ishii, Ryuji Fukuzawa, Koji Muroya, Yukihiro Hasegawa, Toshikatsu Mitsui, Takeshi Sato, Seiji Sato, Junko Igaki |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male endocrine system medicine.medical_specialty Stromal cell Endocrinology Diabetes and Metabolism Ovariectomy 030209 endocrinology & metabolism 03 medical and health sciences Young Adult 0302 clinical medicine Endocrinology Immune system Internal medicine Lipid droplet Testis Medicine Humans Disorder of Sex Development 46 XY Adrenal Hyperplasia Congenital business.industry CD68 Adrenal cortex Macrophages Ovary Infant General Medicine 030104 developmental biology medicine.anatomical_structure CYP17A1 Theca Immunohistochemistry Female business Orchiectomy |
| Zdroj: | European journal of endocrinology. 175(2) |
| ISSN: | 1479-683X |
| Popis: | Objective Congenital lipoid adrenal hyperplasia (lipoid CAH) results in impairment of adrenal and gonadal steroidogenesis caused by STAR mutations. Our previous study revealed upregulation of genes associated with inflammatory or immune response and macrophage infiltration in the adrenal cortex of Star-knockout mice. This study aimed at investigating macrophage infiltration in the gonads from human patients with lipoid CAH. Design This study includes seven patients with lipoid CAH who underwent gonadectomy: two XX women (age, 22 and 40 years) and five XY boys (1 year). Two women with ovarian cysts (32 and 40 years) and six boys with autopsy or tumor (1 year) were examined as controls. Immunohistochemical analysis of their gonads was performed to determine steroidogenic cells by NR5A1 or CYP17A1 and macrophages by IBA1 or CD68. Results An increased number of macrophages infiltrated into the ovaries of lipoid CAH and consisted of two subpopulations: one scattered within and around a layer of theca cells of maturing follicles and the other massively aggregated in the stroma. Abundant cytoplasmic lipid droplets were observed not only in the theca cells but also in the stromal macrophages. There was no significant difference in the number of macrophages in the testicular interstitium between lipoid CAH (95% confidence interval (95% CI: 19.3–47.7 per 0.2mm2) and controls (95% CI: 13.3–25.8 per 0.2mm2) (P=0.10). Conclusions These results demonstrate that macrophages infiltrate the ovaries of lipoid CAH, where the theca cells and the stromal macrophages have abundant cytoplasmic lipid droplets. |
| Databáze: | OpenAIRE |
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