Substrate-dependent inhibition of yeast enolase by fluoride
| Autor: | S.G. Spencer, John M. Brewer |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Phosphoglycerate kinase
biology Chemistry Enolase Inorganic chemistry Biophysics Substrate (chemistry) Active site Cell Biology Saccharomyces cerevisiae Biochemistry Yeast Phosphates chemistry.chemical_compound Fluorides Kinetics Phosphopyruvate Hydratase biology.protein Titration Magnesium Phosphoenolpyruvate carboxykinase Molecular Biology Fluoride |
| Zdroj: | Biochemical and biophysical research communications. 106(2) |
| ISSN: | 0006-291X |
| Popis: | A possibly physiologically significant inhibition of yeast enolase by fluoride occurs in the absence of inorganic phosphate. The inhibition increases with time, is strongly dependent on fluoride concentration and requires substrate and “catalytic” Mg 2+ . The inhibition increases more slowly in the presence of product (phosphoenolpyruvate) than substrate (2-phosphoglycerate). The dependence on fluoride concentration and the spans of substrate analogue displacement titrations suggest the inhibition is produced by two moles of fluoride per active site. |
| Databáze: | OpenAIRE |
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