Reverse line blot hybridization used to identify hemoprotozoa in Minorcan cattle
| Autor: | Sonia Almería, Agustín Estrada-Peña, Olivier Sparagano, J.F. Gutiérrez, David Ferrer, J. Castellà |
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| Rok vydání: | 2002 |
| Předmět: |
Babesia
Cattle Diseases DNA Ribosomal Polymerase Chain Reaction Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology law.invention History and Philosophy of Science law Babesiosis Theileria parasitic diseases medicine Prevalence Animals Minorcan cattle Polymerase chain reaction Babesia bigemina Blot hybridization biology General Neuroscience DNA Protozoan biology.organism_classification medicine.disease Virology Theileria annulata Theileriasis Spain Cattle Seasons |
| Zdroj: | Annals of the New York Academy of Sciences. 969 |
| ISSN: | 0077-8923 |
| Popis: | Piroplasmosis, a tick-borne protozoal disease, is an important disease affecting domestic and wild animals. We performed PCR-based reverse line blot hybridization (RLB) assays on blood samples obtained from 133 cattle exposed to ticks in field conditions in Minorca (Balearic Islands, Spain) in three different seasons. The oligonucleotides used were those for Theileria annulata, T. buffeli, T. taurotragi, T. velifera, Babesia bigemina, B. bovis, B. divergens, and B. major. The RLB technique allowed the simultaneous identification of T. annulata, T. buffeli, B. bigemina, and B. bovis as the piroplasms present in cattle in Minorca. Of the 133 animals, only 4 were not infected by any of the studied parasites. The results indicated endemic piroplasm infection in cattle in Minorca; especially important was the presence of T. annulata. The RLB was highly sensitive and allowed the simultaneous detection and identification of the Theileria and Babesia species in carrier cattle, which cannot be achieved by classical identification methods. |
| Databáze: | OpenAIRE |
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