Simple colorimetric test to quantify exposure to environmental tobacco smoke: occupational health study
| Autor: | Graham Cope, Pamela Nayyar, Roger Holder, Lucy Wilkinson |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Nicotine Saliva Pathology medicine.medical_specialty Passive smoking Metabolite Clinical Biochemistry Urine medicine.disease_cause Risk Assessment Sensitivity and Specificity Tobacco smoke chemistry.chemical_compound Occupational Exposure Environmental health medicine Humans Lung cancer business.industry Environmental Exposure General Medicine medicine.disease chemistry Female Tobacco Smoke Pollution Cotinine business medicine.drug |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 37:795-796 |
| ISSN: | 1758-1001 0004-5632 |
| Popis: | Tobacco smoke is an important environmental contaminant of indoor air. Chronic exposure has been implicated in heart disease and lung cancer in adults and in upper respiratory disease in children. Subjective assessment of exposure, as noted by questionnaires, is dif®cult because of its emotive nature. Therefore, biochemical measurement of biomarkers may provide a more accurate determination of individual exposure. Measurement of nicotine metabolite concentration, such as cotinine, in urine, plasma or saliva is regarded as the approach of choice. A variety of methods are available, such as gas chromatography±mass spectrometry and high-performance liquid chromatography, but they are restricted to the laboratory. Biofeedback about tobacco smoke intake has been shown to be helpful in reducing exposure. To facilitate this feedback we have developed a near-patient test to monitor active smoking using a colorimetric assay to measure nicotine and its metabolites incorporated into a disposable plastic enclosure. We have used this to monitor active smoking in pregnancy and cardiovascular disease. We recently modi®ed the reagents to improve sensitivity to detect nicotine metabolites in saliva in order to develop a chair-side test to monitor smoking in dental practice. Our aim was to determine whether this test could detect exposure to environmental tobacco smoke and to assess whether exposure in an occupational setting correlated with known physiological effects of passive smoking. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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