Treatment of adjuvant arthritis in rats: Vaccination potential of a synthetic nonapeptide from the 65 kDa heat shock protein of mycobacteria
| Autor: | Bernhard Riniker, Xiao-Dong Yang, Jill Gasser, Ulrich Feige |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_treatment Molecular Sequence Data Immunology Arthritis Pharmacology Epitope law.invention Epitopes Bacterial Proteins In vivo law Heat shock protein medicine Animals Immunology and Allergy Amino Acid Sequence Heat-Shock Proteins Antigens Bacterial Mycobacterium bovis biology business.industry Vaccination Immunotherapy biology.organism_classification medicine.disease Arthritis Experimental Peptide Fragments In vitro Rats Rats Inbred Lew Recombinant DNA business Injections Intraperitoneal T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Autoimmunity. 3:11-23 |
| ISSN: | 0896-8411 |
| DOI: | 10.1016/0896-8411(90)90003-b |
| Popis: | Adjuvant arthritis induced by mycobacteria in rats is a widely used model of chronic arthritis. A previously described nonapeptide (Thr-Phe-Gly-Leu-Gln-Leu-Glu-Leu-Thr, amino acid sequence 180-188) from the recombinant 65 kDa heat shock protein of Mycobacterium bovis BCG, which was found to contain a T-cell epitope recognized by both arthritogenic and protective T-cell clones in vitro, has been investigated for its vaccination and therapeutic potential in adjuvant arthritis in rats. The nonapeptide was found not to be arthritogenic, although the T cells from nonapeptide immunized rats cross-react in vitro with mycobacterial antigens. Intraperitoneal administration of 0.1 mg nonapeptide in oil at day -20 or days -2, -1 and 0, resulted in a marked reduction of incidence and severity of adjuvant arthritis. The disease process and severity were also influenced by therapeutic treatment with 0.1 mg nonapeptide injected intraperitoneally at days 7 to 10. Interestingly, subplantar or intravenous application of the nonapeptide had no influence on the disease process. Deletion of the N-terminal threonine led to complete loss of in vivo activity of the nonapeptide. |
| Databáze: | OpenAIRE |
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