Diagnostic utility of flow cytometry based coated‐platelets assay as a biomarker to predict thrombotic or hemorrhagic phenotype in acute stroke
| Autor: | Mahasampath Gowri, Sanjith Aaron, Tulasi Geevar, Joy John Mammen, Rutvi Gautam Dave, Ramya Vijayan, Snehil Kumar, Sukesh C. Nair, Rohit Ninan Benjamin |
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| Rok vydání: | 2021 |
| Předmět: |
Blood Platelets
0301 basic medicine Agonist medicine.medical_specialty Histology medicine.drug_class Phosphatidylserines Gastroenterology Pathology and Forensic Medicine Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Thrombin Internal medicine medicine Humans Platelet Stroke Ischemic Stroke Intracerebral hemorrhage medicine.diagnostic_test business.industry Thrombosis Cell Biology Phosphatidylserine Flow Cytometry Platelet Activation medicine.disease Cross-Sectional Studies Phenotype 030104 developmental biology chemistry 030220 oncology & carcinogenesis Biomarker (medicine) business Biomarkers medicine.drug |
| Zdroj: | Cytometry Part B: Clinical Cytometry. 102:246-253 |
| ISSN: | 1552-4957 1552-4949 |
| DOI: | 10.1002/cyto.b.22026 |
| Popis: | BACKGROUND Coated-platelets are sub-population of platelets "coated" with highly procoagulant proteins and phosphatidylserine that sustains thrombin generation. They are produced upon dual agonist stimulation by collagen and thrombin. This study was conducted to assess if there was any difference in the levels of coated-platelets in patients with primary intracranial hemorrhage (PICH) and ischemic stroke due to large artery atherosclerosis (LAA) as compared to healthy controls, and to see if coated-platelet levels had any influence on the hemorrhagic transformation (HT) of ischemic stroke. METHODS Coated-platelet levels were determined by flow cytometry using fluorescently tagged Annexin V antibody to identify phosphatidylserine exposed on the surface of platelets activated by dual agonists (convulxin and thrombin) in cross-sectional cohort of 75 patients with stroke and 34 controls. RESULTS Patients with PICH (n = 35) had significantly lower coated-platelets than the controls (adjusted mean ± SE, 21.0 ± 1.9% vs. 36.1 ± 1.7%, p |
| Databáze: | OpenAIRE |
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