Isolation and Characterization of Potentially Probiotic Bacterial Strains from Mice: Proof of Concept for Personalized Probiotics
| Autor: | Larissa Sbaglia Celiberto, Roseli Aparecida Pinto, Daniela Cardoso Umbelino Cavallini, Elizeu Antonio Rossi, Bruce A. Vallance |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), BC Children's Hospital and the University of British Columbia |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male colitis Gut flora Inflammatory bowel disease law.invention Probiotic Mice law Lactobacillus Drug Resistance Multiple Bacterial Malondialdehyde Bifidobacterium Nutrition and Dietetics biology Dextran Sulfate 3. Good health Intestines Myeloperoxidase Cytokines lcsh:Nutrition. Foods and food supply Colon IBD personalized probiotic lcsh:TX341-641 Article 03 medical and health sciences Immune system medicine microbiota Animals Colitis Peroxidase Inflammation business.industry Probiotics medicine.disease biology.organism_classification Inflammatory Bowel Diseases microbiota biobank Gastrointestinal Microbiome Mice Inbred C57BL Disease Models Animal 030104 developmental biology Immunology biology.protein business Biomarkers Food Science |
| Zdroj: | Nutrients, Vol 10, Iss 11, p 1684 (2018) Nutrients Volume 10 Issue 11 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2072-6643 |
| Popis: | Modulation of the gut microbiota through the use of probiotics has been widely used to treat or prevent several intestinal diseases. However, inconsistent results have compromised the efficacy of this approach, especially in severe conditions such as inflammatory bowel disease (IBD). The purpose of our study was to develop a personalized probiotic strategy and assess its efficacy in a murine model of intestinal inflammation. Commensal bacterial strains were isolated from the feces of healthy mice and then administered back to the host as a personalized treatment in dextran sodium sulfate (DSS)-induced colitis. Colonic tissues were collected for histological analysis and to investigate inflammatory markers such as Il-1&beta Il-6, TGF-&beta and Il-10, and the enzyme myeloperoxidase as a neutrophil marker. The group that received the personalized probiotic showed reduced susceptibility to DSS-colitis as compared to a commercial probiotic. This protection was characterized by a lower disease activity index and reduced histopathological damage in the colon. Moreover, the personalized probiotic was more effective in modulating the host immune response, leading to decreased Il-1&beta and Il-6 and increased TGF-&beta and Il-10 expression. In conclusion, our study suggests that personalized probiotics may possess an advantage over commercial probiotics in treating dysbiotic-related conditions, possibly because they are derived directly from the host&rsquo s own microbiota. |
| Databáze: | OpenAIRE |
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