Diurnal variation of steroid hormones and their reference intervals using mass spectrometric analysis

Autor: Alan H. DeCherney, K. Welsh, Micah J. Hill, S J Soldin, J Jonklaas, Yesim Ozarda, L Masika, Toral Parikh, Brian Stolze
Přispěvatelé: Uludağ Üniversitesi/Tıp Fakültesi/Tıbbi Biyokimya Anabilim Dalı., Özarda, Yeşim, AAL-8873-2021
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Serum
Male
Steroid blood level
Hydrocortisone
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Physiology
030204 cardiovascular system & hematology
lcsh:Diseases of the endocrine glands. Clinical endocrinology
Assays
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Corticosterone
Clinical Chemistry
Thyrotropin
Chemical Species
Testosterone
Profiles
Progesterone
Endocrinology & metabolism
Priority journal
mass spectrometry
Circadian rhythm
Measurement accuracy
Adrenal insuffiviency
Atmospheric pressure chemical ionization mass spectrometry
Triple quadrupole mass spectrometry
Normal human
Impact
Human experiment
030220 oncology & carcinogenesis
Pediatric reference intervals
Female
medicine.drug
Human
steroids
Steroid hormone
Hormone determination
Cortodoxone
Dehydroepiandrosterone
diurnal variation
Article
Steroid
03 medical and health sciences
LC–MS/MS
Hydroxyprogesterone
Internal Medicine
medicine
Androstenedione
LC-MS/MS
Liquid chromatography-mass spectrometry
Prasterone
lcsh:RC648-665
business.industry
Caliper
Research
Diurnal temperature variation
Sex difference
Cortisone
chemistry
business
Controlled study
Menstrual cycle
Hormone
High performance liquid chromatography
Zdroj: Endocrine Connections
Endocrine Connections, Vol 7, Iss 12, Pp 1354-1361 (2018)
ISSN: 2049-3614
Popis: Objective: Accurate measurement of steroid hormones remains challenging. Mass spectrometry affords a reliable means for quantitating steroid profiles accurately. Our objective was to establish and define (1) the extent of diurnal fluctuations in steroid concentrations that potentially necessitate strict adherence to time of sample acquisition and (2) time-dependent steroid reference intervals. Design: Nine steroid markers were examined in couplets in males and females. Methods: Using isotope dilution high-performance liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis, we developed a multi-steroid profile requiring only a minimal volume of serum (0.1 mL). Couplet (AM and PM) measurements of steroid hormones for 120 healthy females (F) and 62 healthy males (M) were obtained. Patients were recruited from several participating centers. Results: The following diurnal values were noted to be significantly different in both females and males: cortisone, cortisol, corticosterone, 11 deoxycortisol (11 DOC), androstenedione, 17a-hydroxyprogesterone (17 OHP) and dehydroepiandrosterone (DHEA). Testosterone was only found to have significant diurnal variance in males. Progesterone showed no significant difference in AM and PM values for either groups and thus may provide an internal control. Conclusions: When diagnosing endocrine disorders, it is imperative to acknowledge the 24-h diurnal variation of the biochemical steroid markers. We highlight the importance of standardization of collection times and appropriate implementation of reference intervals. Precis: We identify diurnal fluctuations in steroid concentrations with time of day and emphasize the importance of adhering to firm time of sample acquisition. National Center for Advancement of Translational Science (UL1TR001409) United States Department of Health & Human Services National Institutes of Health (NIH) - USA (NCT00428987) National Center for Advancement of Translational Science NIH Intramural Research Grant United States Department of Health & Human Services National Institutes of Health (NIH) - USA NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) (ZIEHD008737) United States Department of Health & Human Services National Institutes of Health (NIH) - USA NIH National Center for Advancing Translational Sciences (NCATS) (UL1TR001409) CLINICAL CENTER (ZIACL010355)
Databáze: OpenAIRE
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