Preparations of intravenous immunoglobulins diminish the number and proinflammatory response of CD14+CD16++ monocytes in common variable immunodeficiency (CVID) patients
| Autor: | Anna Pituch-Noworolska, Danuta Kowalczyk, Monika Baj-Krzyworzeka, Magdalena Rutkowska, Marek Zembala, Magdalena Strach, Anna Szaflarska, Loems Ziegler-Heitbrock, Karolina Bukowska-Strakova, Marzena Lenart, Maciej Siedlar, Tomasz Grodzicki, Kazimierz Weglarczyk |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Lipopolysaccharides Male medicine.medical_treatment CD14 Immunology Lipopolysaccharide Receptors CD16 GPI-Linked Proteins Peripheral blood mononuclear cell Monocytes Immunophenotyping Proinflammatory cytokine Leukocyte Count Young Adult Antigens CD hemic and lymphatic diseases medicine Humans Immunology and Allergy Tumor Necrosis Factor-alpha business.industry Common variable immunodeficiency Monocyte Receptors IgG Antibodies Monoclonal Immunoglobulins Intravenous HLA-DR Antigens Middle Aged medicine.disease Interleukin-12 Interleukin-10 Kinetics Common Variable Immunodeficiency Cytokine medicine.anatomical_structure Leukocytes Mononuclear Female Tumor necrosis factor alpha business |
| Zdroj: | Clinical Immunology. 139:122-132 |
| ISSN: | 1521-6616 |
| DOI: | 10.1016/j.clim.2011.01.002 |
| Popis: | We have studied the effect of intravenous immunoglobulins (IVIG) on monocyte subpopulations and cytokine production in patients with CVID. The absolute number of CD14(+)CD16(++) monocytes decreased on average 2.5-fold 4h after IVIG and after 20h returned to the baseline. The cytokine level in the supernatants of peripheral blood mononuclear cells (PBMC) after ex vivo LPS stimulation demonstrated the >2-fold decrease in TNF production 4h after IVIG. The TNF expression, which is higher in the CD14(+)CD16(++) monocytes, was decreased in these cells by IVIG in 4/7 CVID cases. In vitro exposure of the healthy individuals' monocytes to the IVIG preparation resulted in reduced TNF production, which was overcome by blockade of the FcγRIIB in the CD14(+)CD16(++) CD32B(high) monocytes. Our data suggest that reduction in the number of CD14(+)CD16(++) monocytes and the blockade of their cytokine production via triggering CD32B can contribute to the anti-inflammatory action of IVIG. |
| Databáze: | OpenAIRE |
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