DNA Aptamers for the Characterization of Histological Structure of Lung Adenocarcinoma
| Autor: | Andrey K. Kirichenko, Dmitry V. Veprintsev, Galina S. Zamay, Yury E. Glazyrin, Alexey V. Krat, Anna S. Zamay, Irina V. Garanzha, R. A. Zukov, Valentina L. Grigorieva, Maxim V. Berezovski, Andrey A. Modestov, Andrey A. Barinov, Tatiana N. Zamay, Ana Gargaun, Olga S. Kolovskaya, Marina M. Petrova, Tatiana I. Ivanchenko, Gleb G. Mironov, Sergey S. Bekuzarov |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
histological structure medicine.drug_class medicine.medical_treatment Aptamer DNA-aptamer Biology Monoclonal antibody Targeted therapy 03 medical and health sciences 0302 clinical medicine Drug Discovery medicine Neoplastic transformation Lung cancer lcsh:RM1-950 Cancer medicine.disease lung adenocarcinoma Molecular biology 030104 developmental biology lcsh:Therapeutics. Pharmacology 030220 oncology & carcinogenesis Molecular Medicine Adenocarcinoma Original Article Molecular probe |
| Zdroj: | Molecular Therapy. Nucleic Acids Molecular Therapy: Nucleic Acids, Vol 6, Iss, Pp 150-162 (2017) |
| ISSN: | 2162-2531 |
| Popis: | Nucleic acid aptamers are becoming popular as molecular probes for identification and imaging pathology and, at the same time, as a convenient platform for targeted therapy. Recent studies have shown that aptamers may be effectively used for tumor characterization and as commercially available monoclonal antibodies. Here we present three DNA aptamers binding to whole transformed lung cancer tissues, including tumor cells, connective tissues, and blood vessels. Protein targets have been revealed using affinity purification followed by mass spectrometry analyses, and they have been validated using a panel of correspondent antibodies and 3D imaging of tumor tissues. Each of the proteins targeted by the aptamers is involved in cancer progression and most of them are crucial for lung adenocarcinoma. We propose the use of these aptamers in aptahistochemistry for the characterization of the histological structure of lung adenocarcinoma. The value of the presented aptamers is their application together or separately for indicating the spread of neoplastic transformation, for complex differential diagnostics, and for targeted therapy of the tumor itself as well as all transformed structures of the adjacent tissues. Moreover, it has been demonstrated that these aptamers could be used for intraoperative tumor visualization and margin assessment. Graphical Abstract |
| Databáze: | OpenAIRE |
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