Effects of cadmium on cell viability, trophoblastic development, and expression of low density lipoprotein receptor transcripts in cultured human placental cells
| Autor: | Matthew E. Burow, Michael C. Henson, William J. George, Kenneth F. Swan, Mary Bitner Anderson, Lloyd Jolibois |
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| Rok vydání: | 1999 |
| Předmět: |
inorganic chemicals
medicine.medical_specialty Cell Survival Tetrazolium Salts chemistry.chemical_element Apoptosis DNA Fragmentation Biology Toxicology Andrology Syncytiotrophoblast Pregnancy Placenta Internal medicine medicine Humans RNA Messenger Viability assay Receptor Cells Cultured Differential centrifugation Cadmium Trophoblast Immunohistochemistry Trophoblasts Thiazoles Endocrinology medicine.anatomical_structure Receptors LDL chemistry LDL receptor Female |
| Zdroj: | Reproductive Toxicology. 13:473-480 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/s0890-6238(99)00041-6 |
| Popis: | Previously, we have demonstrated that cadmium inhibits progesterone release in cultured human trophoblast cells. In the present study, we investigated potential mechanism(s) by which cadmium may elicit this effect. Cytotrophoblasts were obtained via enzymatic dispersion, purified by density gradient centrifugation, and cultured with increasing concentrations of cadmium. Cadmium-induced suppression of progesterone release seemed to be independent of cell death, as no significant decline in viability was observed with cadmium treatment. Further, immunocytochemical localization of cellular boundaries and nuclei indicated approximately 94% syncytial maturity was attained by both untreated and cadmium-treated cells, demonstrating that cadmium did not inhibit syncytial development. However, the abundance of LDL receptor (LDL-R) mRNA transcripts, as determined by competitive RT-PCR, was reduced (P < 0.05) by cadmium exposure in an apparent dose-dependent manner. Thus, the LDL-R, by which cholesterol substrate is supplied to the syncytiotrophoblast, is one site at which cadmium may interfere with placental progesterone production. |
| Databáze: | OpenAIRE |
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