Collection of Leukocytes, Fibroblasts, and Collagen Within an Implantable Reservoir Tube During Tissue Repair
| Autor: | William J. Lindblad, T.C. Smith, J.C. Kim, Robert F. Diegelmann, I.K. Cohen, T.M. Harris |
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| Rok vydání: | 1987 |
| Předmět: |
Male
Pathology medicine.medical_specialty Immunology Cell Connective tissue Lumen (anatomy) Inflammation Cell Separation Biology Leukocytes medicine Animals Immunology and Allergy Fibroblast Wound Healing Rats Inbred Strains Exudates and Transudates Prostheses and Implants Cell Biology Fibroblasts Tissue repair Rats medicine.anatomical_structure Wound fluid Collagen medicine.symptom Wound healing |
| Zdroj: | Journal of Leukocyte Biology. 42:667-672 |
| ISSN: | 1938-3673 0741-5400 |
| Popis: | An implantable chamber consisting of a small reservoir and a perforated segment of silicone tubing has been developed for the collection of leukocytes, fibroblasts, and collagen to analyze inflammatory components and fibroplasia during tissue repair. Using aseptic techniques, these sterile chambers were placed into subcutaneous pockets on the backs of Sprague-Dawley rats with cells obtained in daily aspirates for 14 d. Differential cell counts were made by using aliquots from the wound fluid. The aspirated cells represented the characteristic, sequential influx of neutrophils, inflammatory macrophages, lymphocytes, activated macrophages, and fibroblasts documented in other models of tissue repair. On d 14, the connective tissue within the lumen of the silicone tube was removed and analyzed for collagen synthesis by measuring 3H-proline incorporation into collagenase-sensitive protein. This new device for studying wound healing provides a convenient means to harvest cells, fluid, and tissue for cellular, humoral, and biochemical analyses of tissue repair. |
| Databáze: | OpenAIRE |
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