Extract and fraction from Ulmus wallichiana Planchon promote peak bone achievement and have a nonestrogenic osteoprotective effect
| Autor: | K. R. Arya, Avinash Kumar, Kunal Sharan, Abdul Malik Tyagi, Lakshmi Manickavasagam, Gaurav Swarnkar, Girish Kumar Jain, Preeti Rawat, Naibedya Chattopadhyay, Rakesh Maurya, Manmeet Kumar, Geet Kumar Nagar, Jawed A. Siddiqui |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Ovariectomy Ulmus Fraction (chemistry) law.invention Rats Sprague-Dawley Weight-Bearing Bone Density Osteogenesis law Internal medicine Animals Humans Medicine Glycosides Chromatography High Pressure Liquid Osteoporosis Postmenopausal Flavonoids Osteoblasts Plant Stems biology Traditional medicine business.industry Obstetrics and Gynecology Ulmus wallichiana biology.organism_classification Rats carbohydrates (lipids) Sprague dawley Endocrinology Plant Bark Ovariectomized rat Female lipids (amino acids peptides and proteins) Plant Preparations business Phytotherapy Biomarkers |
| Zdroj: | Menopause. 17:393-402 |
| ISSN: | 1072-3714 |
| DOI: | 10.1097/gme.0b013e3181bfae38 |
| Popis: | This study aimed to determine the skeletal effects of total ethanolic extract (TEE) and its butanolic fraction (BF) from the stem-bark of Ulmus wallichiana, which is rich in C-glycosylated flavonoids, in growing rats (for peak bone [PB] achievement) and in ovariectomized (OVx) rats (for menopausal bone loss).TEE (750 mg kg(-1) d(-1)) and BF (50 mg kg(-1) d(-1)) were given orally for 10 weeks to weaning female Sprague-Dawley rats and for 12 weeks to adult OVx rats of the same strain, respectively. In studies with OVx rats, sham operated + vehicle, OVx + 17beta-estradiol, and OVx + vehicle groups served as various controls. Bone mineral density (BMD), biomechanical strength, bone histology, formations of osteoprogenitor cells, osteoid formation, and bone turnover/resorption markers were studied. Bioactive marker compounds in TEE and BF were analyzed by high-performance liquid chromatography. One-way analysis of variance was used to test significance of effects.In growing rats, both TEE and BF increased BMD, bone strength, and bone formation rate, suggesting higher PB achievement. OVx rats treated with either TEE or BF exhibited increased BMD at various anatomical positions and improved bone strength and trabecular architecture compared with the OVx + vehicle group. Serum osteocalcin and urinary type 1 collagen degradation product levels in OVx rats treated with either TEE or BF were significantly lower than those of the OVx + vehicle group. Neither TEE nor BF exhibited uterine estrogenicity. Analysis of marker compounds revealed significant enrichment of two bioactive markers in BF over TEE.Derived from U wallichiana, BF at much a lower dose than TEE was effective in PB achievement and prevention of OVx-induced bone loss. |
| Databáze: | OpenAIRE |
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