5'-Ribonucleotides production using 5'-phosphodiesterase from spent malt roots
| Autor: | Szymon Macieja, Edson Marcelino Alves, Joyce Faria de Souza, Pedro de Oliva Neto |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
RNA hydrolysis
03 medical and health sciences Hydrolysis 0404 agricultural biotechnology Barley malt rootlets Spent brewer’s yeast TX341-641 Food science 030304 developmental biology 0303 health sciences Nutrition. Foods and food supply business.industry Barley Malt Chemistry Extraction (chemistry) Enzyme kinetics Phosphodiesterase 04 agricultural and veterinary sciences 040401 food science Yeast Bioprocess optimization Brewing business Brewing industry wastes Food Science |
| Zdroj: | Brazilian Journal of Food Technology v.24 2021 Brazilian Journal of Food Technology Instituto de Tecnologia de Alimentos (ITAL) instacron:ITAL Brazilian Journal of Food Technology, Vol 24 (2021) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1981-6723 |
| Popis: | Made available in DSpace on 2021-06-25T10:29:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01. Added 1 bitstream(s) on 2021-07-15T15:22:45Z : No. of bitstreams: 1 S1981-67232021000100416.pdf: 1575563 bytes, checksum: 57a59a307b40141a3cdea9e9ba92b0c3 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) 5'-ribonucleotides are high value-added molecules widely used in the food and pharmaceutical industries because of their bioactive properties. The present work aims to produce a composition of 5’-ribonucleotides using spent brewer’s yeast as cheap source of RNA, and barley malt rootlets as cheap source of 5'-phosphodiesterase (5'-PDE). This is a very promising and innovative strategy because both spent yeast and malt rootles are residues of the brewing process and are closely linked in a cycle that until now is not yet commercially exploited due to lack of studies. Our results showed that extraction of 5’-PDE was mainly influenced by the fineness of the rootlets and amount of extraction solvent (water). The main molecules formed during RNA hydrolysis were 5’-ribonucleotides, which represented 85.86% of the total hydrolyzed molecules. Finally, the results of the proposed approach can generate a new perspective for the brewing industry regarding the management of its wastes, generating from them products of high added value and with a wide range of applications. Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de Ciências e Letras de Assis Departamento de Biotecnologia Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de Ciências e Letras de Assis Departamento de Biotecnologia FAPESP: 2016/08886-6 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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