Immunization with a recombinant bacillus calmette-guerin strain confers protective th1 immunity against the human metapneumovirus
| Autor: | Alexis M. Kalergis, Pablo F. Céspedes, Susan M. Bueno, Roberto S. Gómez, Christian E. Palavecino |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
T-Lymphocytes
viruses Immunology Virus Cell Line Mice Viral Proteins Immune system Human metapneumovirus Immunity Animals Humans Immunology and Allergy Lung Immunity Cellular Vaccines Synthetic Mycobacterium bovis Paramyxoviridae Infections biology virus diseases Th1 Cells Phosphoproteins biology.organism_classification Adoptive Transfer Antibodies Bacterial Virology respiratory tract diseases Disease Models Animal Immunization Immunoglobulin G BCG Vaccine biology.protein Cytokines bacteria Metapneumovirus Antibody Tuberculosis vaccines |
| Zdroj: | JOURNAL OF IMMUNOLOGY Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | Along with the human respiratory syncytial virus (hRSV), the human metapneumovirus (hMPV) is one of the leading causes of childhood hospitalization and a major health burden worldwide. Unfortunately, owing to an inefficient immunological memory, hMPV infection provides limited immune protection against reinfection. Furthermore, hMPV can induce an inadequate Th2 type immune response that causes severe lung inflammation, leading to airway obstruction. Similar to hRSV, it is likely that an effective clearance of hMPV would require a balanced Th1 type immunity by the host, involving the activation of IFN-γ–secreting T cells. A recognized inducer of Th1 immunity is Mycobacterium bovis bacillus Calmette–Guérin (BCG), which has been used in newborns for many decades and in several countries as a tuberculosis vaccine. We have previously shown that immunization with BCG strains expressing hRSV Ags can induce an efficient immune response that protects against this virus. In this study, we show that immunization with rBCG strains expressing the phosphoprotein from hMPV also can induce protective Th1 immunity. Mice immunized with rBCG were protected against weight loss, airway inflammation, and viral replication in the lungs after hMPV infection. Our rBCG vaccine also induced the activation of hMPV-specific T cells producing IFN-γ and IL-2, which could protect from hMPV infection when transferred to recipient mice. These data strongly support the notion that rBCG induces protective Th1 immunity and could be considered as an efficient vaccine against hMPV. |
| Databáze: | OpenAIRE |
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