Evaluation of strontium-containing PCL-PDIPF scaffolds for bone tissue engineering: In vitro and in vivo studies

Autor: Juan Manuel Fernández, Agustina Berenice Lino, Antonio Desmond McCarthy
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
POLYDIISOPROPYL FUMARATE
Polymers
Biología
Biocompatible Materials
02 engineering and technology
Bone tissue
BONE REGENERATION
Mice
RAW 264.7 macrophages
Fumarates
POLY-Ε-CAPROLACTONE
purl.org/becyt/ford/3.4 [https]
Cells
Cultured
chemistry.chemical_classification
Tissue Scaffolds
RAW 264.7 MACROPHAGES
Bone regeneration
medicine.anatomical_structure
purl.org/becyt/ford/3 [https]
inorganic chemicals
Stromal cell
Cell Survival
Polyesters
0206 medical engineering
Biomedical Engineering
chemistry.chemical_element
Calcium
Nitric Oxide
Bone and Bones
Divalent
STRONTIUM
In vivo
medicine
Animals
Ciencias Exactas
Polydiisopropyl fumarate
Tissue Engineering
Bone marrow stromal cells
technology
industry
and agriculture
Poly-ε-caprolactone
BONE MARROW STROMAL CELLS
020601 biomedical engineering
In vitro
Rats
RAW 264.7 Cells
chemistry
Strontium
Biophysics
Bone marrow
Stromal Cells
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
SEDICI (UNLP)
Universidad Nacional de La Plata
instacron:UNLP
DOI: 10.1007/s10439-018-02183-z
Popis: Bone tissue engineering (BTE) has the general objective of restoring and improving damaged bone. A very interesting strategy for BTE is to combine an adequate polymeric scaffold with an osteoinductive compound. Strontium is a divalent cation that can substitute calcium in hydroxyapatite and induce both anabolic and anti-catabolic effects in bone. On the other hand, systemic increases in Sr²⁺ levels can provoke adverse cardiovascular effects. In the present study we have developed a compatibilized blend of poly-ε-caprolactone (PCL) and polydiisopropyl fumarate (PDIPF) enriched with 1% or 5% Sr²⁺ and evaluated the applicability of these biomaterials for BTE, both in vitro and in vivo. In vitro, whereas Blend + 5% Sr²⁺ was pro-inflammatory and anti-osteogenic, Blend + 1% Sr²⁺ released very low quantities of the cation; was not cytotoxic for cultured macrophages; and showed improved osteocompatibility when used as a substratum for primary cultures of bone marrow stromal cells. In vivo, implants with Blend + 1% Sr²⁺ significantly increased bone tissue regeneration and improved fibrous bridging (vs. Blend alone), while neither inducing a local inflammatory response nor increased serum levels of Sr²⁺. These results indicate that our compatibilized blend of PCL-PDIPF enriched with 1% Sr²⁺ could be useful for BTE.
Facultad de Ciencias Exactas
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Databáze: OpenAIRE
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