Cepharanthine alleviates liver injury in a rodent model of limb ischemia–reperfusion
| Autor: | Ming-Chang Kao, Yin Kuang Chang, Chun Jen Huang, Su Cheng Huang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.medical_treatment Prostaglandin Hindlimb Benzylisoquinolines Antioxidants Rats Sprague-Dawley Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Cepharanthine Animals Medicine Aspartate Aminotransferases Liver injury biology business.industry Liver Diseases Anti-Inflammatory Agents Non-Steroidal Alanine Transaminase Extremities General Medicine medicine.disease Rats Surgery Disease Models Animal 030104 developmental biology Anesthesiology and Pain Medicine Endocrinology Cytokine chemistry Alanine transaminase Reperfusion Injury 030220 oncology & carcinogenesis biology.protein Lipid Peroxidation business Reperfusion injury |
| Zdroj: | Acta Anaesthesiologica Taiwanica. 54(1):11-15 |
| ISSN: | 1875-4597 |
| DOI: | 10.1016/j.aat.2015.11.004 |
| Popis: | Limb ischemia-reperfusion (I/R) causes remote organ injury (e.g., liver injury). Oxidation and inflammation are crucial mechanisms. We investigated the effects of cepharanthine, a potent antioxidative and anti-inflammatory drug, on alleviating liver injury induced by limb I/R.Twenty-four adult male Sprague-Dawley rats were randomized to receive sham operation (Sham), Sham plus cepharanthine, I/R, or I/R plus cepharanthine and designated as the Sham, Sham+Cep, I/R, or I/R+Cep group, respectively (n = 6 in each group). I/R was induced by applying rubber band tourniquets high around each hind limb for 3 hours followed by reperfusion for 24 hours.The plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of the Sham and Sham+Cep groups were low, and the levels of AST and ALT of the I/R group were significantly higher than those of the Sham group (both p0.001). By contrast, the AST and ALT of the I/R+Cep group were significantly lower than those of the I/R group (both p0.001). The hepatic levels of nitric oxide (NO), malondialdehyde (MDA), macrophage inflammatory protein 2 (MIP-2), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2) of the Sham and Sham+Cep groups were also low. As expected, the NO, MDA, MIP-2, IL-6, and COX-2/PGE2 of the I/R group were significantly higher than those of the Sham group (all p0.001). By contrast, the NO, MDA, MIP-2, IL-6, and COX-2/PGE2 of the I/R+Cep group were significantly lower than those of the I/R group (all p0.05).Cepharanthine alleviates liver injury in a rodent model of limb I/R. The mechanisms may involve reducing oxidation and inflammation. |
| Databáze: | OpenAIRE |
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