DNA barcoding for identification of sand fly species (Diptera: Psychodidae) from leishmaniasis-endemic areas of Peru
| Autor: | Chukwunonso O. Nzelu, Henrry H. Yañez-Trujillano, Ken Katakura, Carlos E. Holguín-Mauricci, Martín J. Arrunátegui-Jiménez, Deysi Luna-Caipo, Máximo F. Lañas-Rosas, Yoshihisa Hashiguchi, Abraham G. Cáceres, Hirotomo Kato |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Endemic Diseases
Veterinary (miscellaneous) Molecular Sequence Data Population Zoology Disease Vectors DNA barcoding Intraspecific competition Electron Transport Complex IV parasitic diseases Peru DNA barcode Animals DNA Barcoding Taxonomic Humans Psychodidae education leishmaniasis education.field_of_study biology fungi Interspecific competition biology.organism_classification Genetic divergence Infectious Diseases Phlebotomus Insect Science Vector (epidemiology) Parasitology Subgenus sand flies vector |
| Zdroj: | Acta Tropica. 145:45-51 |
| ISSN: | 0001-706X |
| Popis: | Phlebotomine sand flies are the only proven vectors of leishmaniases, a group of human and animal diseases. Accurate knowledge of sand fly species identification is essential in understanding the epidemiology of leishmaniasis and vector control in endemic areas. Classical identification of sand fly species based on morphological characteristics often remains difficult and requires taxonomic expertise. Here, we generated DNA barcodes of the cytochrome c oxidase subunit 1 (COI) gene using 159 adult specimens morphologically identified to be 19 species of sand flies, belonging to 6 subgenera/species groups circulating in Peru, including the vector species. Neighbor-joining (NJ) analysis based on Kimura 2-Parameter genetic distances formed non-overlapping clusters for all species. The levels of intraspecific genetic divergence ranged from 0 to 5.96%, whereas interspecific genetic divergence among different species ranged from 8.39 to 19.08%. The generated COI barcodes could discriminate between all the sand fly taxa. Besides its success in separating known species, we found that DNA barcoding is useful in revealing population differentiation and cryptic diversity, and thus promises to be a valuable tool for epidemiological studies of leishmaniasis. Copyright (C) 2015 Published by Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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