Analysis of allelic differential expression in the human genome using allele-specific serial analysis of gene expression tags
| Autor: | Lilian Campos Pires, Pedro A. F. Galante, Anamaria A. Camargo, Anna Christina M. Salim, Maria Cecília Costa, Daniel O. Vidal, Cibele Masotti, Sandro J. de Souza, Jorge Estefano Santana de Souza |
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| Rok vydání: | 2011 |
| Předmět: |
Candidate gene
DNA Complementary Genotype Genetic Linkage Sequence analysis Biology Genome Genetics Humans RNA Messenger Serial analysis of gene expression Molecular Biology Gene Alleles Gene Library Expressed Sequence Tags Expressed sequence tag Genome Human Gene Expression Profiling fungi Chromosome Mapping Sequence Analysis DNA General Medicine Gene expression profiling SAGE Library Microsatellite Repeats Biotechnology |
| Zdroj: | Genome. 54:120-127 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/g10-103 |
| Popis: | Recent reports have demonstrated that a significant proportion of human genes display allelic differential expression (ADE). ADE is associated with phenotypic variability and may contribute to complex genetic diseases. Here, we present a computational analysis of ADE using allele-specific serial analysis of gene expression (SAGE) tags representing 1295 human genes. We identified 472 genes for which unequal representation (>3-fold) of allele-specific SAGE tags was observed in at least one SAGE library, suggesting the occurrence of ADE. For 235 out of these 472 genes, the difference in the expression level between both allele-specific SAGE tags was statistically significant (p < 0.05). Eleven candidate genes were then subjected to experimental validation and ADE was confirmed for 8 out of these 11 genes. Our results suggest that at least 25% of the human genes display ADE and that allele-specific SAGE tags can be efficiently used for the identification of such genes. |
| Databáze: | OpenAIRE |
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