Tapping-mode atomic force microscopy in fluid of hydrated extracellular matrix
| Autor: | Terenzio Congiu, Mario Raspanti, Stefano Guizzardi |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Materials science Atomic force microscopy Water macromolecular substances Anatomy Microscopy Atomic Force Fibril Extracellular Matrix Rats Tendon Collagen fibril Tendons Extracellular matrix Coupling (electronics) medicine.anatomical_structure Phase (matter) Microfibrils medicine Biophysics Ultrastructure Animals Female Molecular Biology |
| Popis: | Fragments of native, hydrated rat tail tendon were imaged by tapping-mode atomic force microscopy while immersed in fluid. The specimens were soft and sensitive to the operating parameters, and with minimal imaging pressure the collagen fibrils appeared covered by irregular blobs or by filamentous material. A slight increase in pressure caused the underlying fibril surface to appear, with an evident D-period, gap- and overlap-zones and three intraperiod ridges. Fibrils often ran parallel and in phase, implying some coupling mechanism. Longitudinal subfibrils, 8–9 nm thick, occasionally appeared. The simultaneous acquisition of the ‘tapping amplitude’ along with the usual ‘height’ channel clearly confirmed the presence of longitudinal subfibrils, indicative of the inner architecture of the fibril. |
| Databáze: | OpenAIRE |
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