An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules
| Autor: | C. Vila-Sanjurjo, Saaketh Desai, Francisco M. Goycoolea, Christoph Engwer, Xiaofei Qin |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Drug Compounding Dispersity Nanoparticle tracking analysis Nanotechnology Biosensing Techniques 02 engineering and technology Acyl-Butyrolactones Nanocapsules Chitosan 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry Dynamic light scattering Escherichia coli Particle Size Physical and Theoretical Chemistry biology Chemistry Quorum Sensing Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Quorum sensing Spectrometry Fluorescence 030104 developmental biology Chemical engineering 0210 nano-technology Biosensor Bacteria Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 149:358-368 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2016.10.031 |
| Popis: | We examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ∼114–155 nm, polydispersity ∼0.127, and ζ-potential ∼+50 mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a “stoichiometric” ratio of ∼80 NC/bacterium. By SEM it was possible to image the aggregation between NC and bacteria. Moreover, we developed a custom in silico platform to simulate the behavior of particles with diameters of 150 nm and ζ-potential of +50 mV on the bacterial surface. We computed the detailed force interactions between NC-NC and NC-bacteria and found that a maximum number of 145 particles might interact at the bacterial surface. Additionally, we found that the “stoichiometric” ratio of NC and bacteria has a strong influence on the bacterial behavior and influences the quorum sensing response, particularly due to the aggregation driven by NC. |
| Databáze: | OpenAIRE |
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