TIMP-2 Modulates Neointimal Formation in Experimental ePTFE Arterial Grafts
| Autor: | Antonino Cavallaro, Giuseppe Cavallaro, Giorgio De Toma, Alessandra Cucina, Mosiello G, Andrea Polistena, Bruto Randone, Pierpaolo Coluccia |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Neointima Pathology medicine.medical_specialty Reconstructive surgery TIMP-2 neointima arterial graft Antineoplastic Agents Matrix Metalloproteinase Inhibitors Matrix (biology) Muscle Smooth Vascular Extracellular matrix Postoperative Complications medicine.artery medicine Animals Thoracic aorta Aorta Abdominal Polytetrafluoroethylene Tissue Inhibitor of Metalloproteinase-2 business.industry Abdominal aorta Antibodies Monoclonal Immunohistochemistry Blood Vessel Prosthesis Extracellular Matrix Rats Surgery medicine.anatomical_structure Rats Inbred Lew eptfe grafts extracellular matrix timp-2 Matrix Metalloproteinase 2 Endothelium Vascular Tunica Intima business Extracellular Matrix Degradation Artery |
| Zdroj: | Journal of Surgical Research. 137:122-129 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2006.07.038 |
| Popis: | Background In vascular reconstructive surgery, myointimal hyperplasia contributes to the adverse outcome of synthetic grafts. This phenomenon is because of unregulated extracellular matrix degradation and remodeling, and excessive smooth muscle cell proliferation and migration. Matrix metallopreoteinase 2 (MMP-2) is known as an important contributor to these events. The aims of our study was to investigate the effects of selective MMP-2 inhibitor (TIMP-2) in endothelialization rate, SMC proliferation, and myointimal hyperplasia in experimental ePTFE arterial grafts. Methods In 20 male Lewis rats, a 1-cm long ePTFE graft has been inserted at the level of the abdominal aorta. Animals were randomized in two groups (10 animals each): group A received six subcutaneous inoculations of TIMP-2 (2.5 μg) after surgery, group B received only the vehicle of TIMP-2. Results Neointimal thickness, as well as SMC density, were augmented in group B, whereas endothelial cells density was augmented in group A, and these findings were statistically significant. In group A SMC were better organized, just like SMC of thoracic aorta. In group B SMC were no organized. Furthermore, anti-TIMP-2 and anti-MMP-2 coloration revealed higher levels of TIMP-2 and lower levels of MMP-2 in group A versus group-B. Conclusions Use of TIMP-2 affects the neointimal formation of experimental e-PTFE arterial grafts, leading to a better-organized neointima, with improved endothelialization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect