Tumor-related gene changes in immunosuppressive Syrian hamster cholangiocarcinoma
| Autor: | Porntip Laummaunwai, Ratchadawan Aukkanimart, Thidarut Boonmars, Pranee Sriraj, Pakkayanee Sudsarn, Nadchanan Wonkchalee, Amornrat Juasook |
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| Rok vydání: | 2012 |
| Předmět: |
endocrine system
Cancer Research Pathology medicine.medical_specialty animal diseases Prednisolone Anti-Inflammatory Agents Hamster Biology Opisthorchiasis Pathology and Forensic Medicine Dimethylnitrosamine Cholangiocarcinoma Cytokeratin Superoxide Dismutase-1 Cricetinae Proliferating Cell Nuclear Antigen parasitic diseases medicine Animals Opisthorchis viverrini Keratin-19 Analysis of Variance Mesocricetus Interleukin-6 Superoxide Dismutase General Medicine medicine.disease biology.organism_classification Catalase Proliferating cell nuclear antigen Gene Expression Regulation Neoplastic Bile Ducts Intrahepatic Oncology Bile Duct Neoplasms Cyclooxygenase 2 biology.protein Immunohistochemistry medicine.drug |
| Zdroj: | Pathology oncology research : POR. 19(4) |
| ISSN: | 1532-2807 |
| Popis: | The results of a previous study demonstrated that prednisolone enhanced cholangiocarcinogenesis. Therefore, to clarify molecular changes during immunosuppressive cholangiocarcinogenesis, Syrian hamsters were divided into 8 groups: uninfected controls; immunosuppressed Syrian hamsters using prednisolone (P); normal Syrian hamsters administered N-nitrosodimethylamine (ND); immunosuppressed Syrian hamsters administered N-nitrosodimethylamine (NDis); normal Syrian hamsters infected with Opisthorchis viverrini (OV); immunosuppressed Syrian hamsters infected with O. viverrini (OVis); normal Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCA); and immunosuppressed Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCAis). Syrian hamster livers were used for analysis of tumor-related gene expression and immunohistochemistry through cytokeratin 19 (CK19) and proliferating cell nuclear antigen (PCNA) staining. The tumor-related gene expression results show that CCAis groups at all time points exhibited upregulation of COX-2, IL-6, SOD1, CAT and iNOS and downregulation of p53, which correlated with the predominant expression of CK19 and PCNA in liver tissue. These results suggest that prednisolone enhances cholangiocarcinoma development, which was confirmed by molecular changes. |
| Databáze: | OpenAIRE |
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