An analysis of multiplex-PCR in the detection of BCR-ABL transcripts in hematological disorders
| Autor: | Cecilia M. Rodriguez, Carlos E. Argaraña, Darío Sastre, Viviana Heller, Mónica Gallo, Enrique Fernández |
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| Rok vydání: | 2007 |
| Předmět: |
Hematological disorders
Acute leukemia lcsh:QH426-470 Acute myeloblastic leukemia Specific detection Lymphoblastic Leukemia Ph chromosome Myeloid leukemia multiplex-PCR Biology medicine.disease lcsh:Genetics PCR chronic myeloid leukemia hemic and lymphatic diseases Immunology Multiplex polymerase chain reaction Genetics medicine BCR-ABL neoplasms Molecular Biology Nested polymerase chain reaction |
| Zdroj: | Genetics and Molecular Biology, Volume: 30, Issue: 3, Pages: 520-523, Published: 2007 Genetics and Molecular Biology, Vol 30, Iss 3, Pp 520-523 (2007) |
| ISSN: | 1415-4757 |
| Popis: | In this work, we describe the advantages of multiplex-PCR in the specific detection of BCR-ABL transcripts in different hematological disorders and its sensitivity compared to nested PCR. Fifty-three patients were studied for the presence of BCR-ABL transcripts: 24 patients with chronic myeloid leukemia (CML), 20 with acute leukemia (AL), and 9 patients with other hematological disorders. A variant rearrangement (b3a3) was found in a single case of CML (4.2%). Four out of the 20 patients with AL (20.0%) (14 adults, 6 children) were bcr-abl(+), and in this group three cases were classified as B-acute lymphoblastic leukemia (B-ALL), and one as acute myeloblastic leukemia (AML). Two of the three patients with B-ALL were positive for b2a2 and the other one for e1a2, while in the BCR-ABL(+)AML patients a b3a2 rearrangement was observed. In conclusion, multiplex-PCR allows rapid, specific and simultaneous detection of different types of BCR-ABL transcripts in CML and ABL-BCR(+)AL. A full correlation was observed when multiplex-PCR was compared with nested PCR. |
| Databáze: | OpenAIRE |
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