Rapid HPLC measurement of buflomedil in plasma in poisoning cases
| Autor: | I. Forfar-Bares, F. Pehourcq, C. Jarry |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pharmacology
Pyrrolidines Chromatography Poisoning Vasodilator Agents Clinical Biochemistry Extraction (chemistry) HPLC measurement Reproducibility of Results General Medicine Plasma Reference Standards Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Human plasma Buflomedil Calibration Drug Discovery Biological fluids Humans Diethyl ether Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Biomedical Chromatography. 16:482-485 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.189 |
| Popis: | A rapid high-performance liquid chromatographic method for the determination of buflomedil in human plasma is described. It requires a single liquid–liquid extraction step from 1 mL of plasma with diethyl ether followed by chromatography on a Nova Pak C18 reversed-phase column and detection by ultaviolet light. Metoclopramide was used as internal standard. The method is sensitive with a quantification limit at 500 ng/mL. It was used for the determination of buflomedil in biological fluids in poisoning cases. Copyright © 2002 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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