Periosteum‐derived mesenchymal progenitor cells in engineered implants promote fracture healing in a critical‐size defect rat model
| Autor: | Gloria Abizanda, Emma Muiños-López, Tania López-Martínez, Elena M. De-Juan-Pardo, Purificación Ripalda-Cemboráin, Felipe Prosper, Kai Stuckensen, M.R. Elizalde, Ion Andreu, Ana B. González-Gil, José Valdés-Fernández, Jürgen Groll, Froilán Granero-Moltó, María Flandes-Iparraguirre, José María Lamo-Espinosa |
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| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty 0206 medical engineering Nonunion Biomedical Engineering Medicine (miscellaneous) 02 engineering and technology Bone healing Rats Sprague-Dawley Biomaterials 03 medical and health sciences Periosteum medicine Animals Progenitor cell Bone regeneration 030304 developmental biology Bioprosthesis Fracture Healing 0303 health sciences Tissue Engineering Chemistry Mesenchymal stem cell Mesenchymal Stem Cells medicine.disease 020601 biomedical engineering Rats CTL medicine.anatomical_structure Cortical bone Femoral Fractures |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 13:742-752 |
| ISSN: | 1932-7005 1932-6254 |
| Popis: | An attractive alternative to bone autografts is the use of autologous mesenchymal progenitor cells (MSCs) in combination with biomaterials. We compared the therapeutic potential of different sources of mesenchymal stem cells in combination with biomaterials in a bone nonunion model. A critical‐size defect was created in Sprague–Dawley rats. Animals were divided into six groups, depending on the treatment to be applied: bone defect was left empty (CTL); treated with live bone allograft (LBA); hrBMP‐2 in collagen scaffold (CSBMP2); acellular polycaprolactone scaffold (PCL group); PCL scaffold containing periosteum‐derived MSCs (PCLPMSCs) and PCL containing bone marrow‐derived MSCs (PCLBMSCs). To facilitate cell tracking, both MSCs and bone graft were isolated from green fluorescent protein (GFP)‐transgenic rats. CTL group did not show any signs of healing during the radiological follow‐up (n = 6). In the LBA group, all the animals showed bone bridging (n = 6) whereas in the CSBMP2 group, four out of six animals demonstrated healing. In PCL and PCLPMSCs groups, a reduced number of animals showed radiological healing, whereas no healing was detected in the PCLBMSCs group. Using microcomputed tomography, the bone volume filling the defect was quantified, showing significant new bone formation in the LBA, CSBMP2, and PCLPMSCs groups when compared with the CTL group. At 10 weeks, GFP positive cells were detected only in the LBA group and restricted to the outer cortical bone in close contact with the periosteum. Tracking of cellular implants demonstrated significant survival of the PMSCs when compared with BMSCs. In conclusion, PMSCs improve bone regeneration being suitable for mimetic autograft design. |
| Databáze: | OpenAIRE |
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