Adult hemoglobins are synthesized in erythroid colonies in vitro derived from murine circulating hemopoietic progenitor cells during embryonic development
Autor: | David H.K. Chui, David H. Carr, Bryan J. Clarke, Peter M. C. Wong |
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Rok vydání: | 1982 |
Předmět: |
Multidisciplinary
Embryonic Fluid Hemoglobins Abnormal Cellular differentiation Embryogenesis Cell Differentiation Biology Hematopoietic Stem Cells Embryonic stem cell Molecular biology Hemoglobins Mice Haematopoiesis medicine.anatomical_structure Liver medicine Animals Erythropoiesis Progenitor cell Yolk sac Cells Cultured Research Article Yolk Sac |
Zdroj: | Proceedings of the National Academy of Sciences. 79:2952-2956 |
ISSN: | 1091-6490 0027-8424 |
DOI: | 10.1073/pnas.79.9.2952 |
Popis: | Circulating peripheral blood cells and disaggregated yolk sac cells were obtained from normal mouse embryos as early as day 9 of gestation, prior to the formation of the fetal liver. These were cultured in vitro in plasma clots or methylcellulose, in the presence of either embryonic fluid or adult spleen cell-conditioned medium, with or without added erythropoietin. Large erythroid colonies were observed by the sixth day of culture. In all instances, these erythroid colonies synthesized adult hemoglobins. These results indicate that erythroid progenitor cells committed to adult hemoglobin synthesis are present in early embryonic circulation. |
Databáze: | OpenAIRE |
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