A molecular comparison of T lymphocyte populations infiltrating the liver and circulating in the blood of patients with chronic hepatitis B: Evidence for antigen-driven selection of a public complementarity-determining region 3 (CDR3) motif
| Autor: | Agnieszka M. Lichanska, Andrew Ladhams, Graham Cooksley, Garwin K. Sing, Dili Li, Tom Macnaughton, L. A. Butterworth, Xiaohua Chen |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male medicine.medical_specialty Adolescent T-Lymphocytes T cell Molecular Sequence Data Complementarity determining region CD8-Positive T-Lymphocytes Biology medicine.disease_cause Hepatitis B Chronic Antigen Internal medicine medicine Humans Amino Acid Sequence Antigens Hepatitis B virus Blood Cells Base Sequence Hepatology T-cell receptor T lymphocyte Middle Aged Complementarity Determining Regions Clone Cells medicine.anatomical_structure Liver Immunology Female Viral load |
| Zdroj: | Hepatology. 33:1288-1298 |
| ISSN: | 0270-9139 |
| Popis: | Despite a large number of T cells infiltrating the liver of patients with chronic hepatitis B, little is known about their complexity or specificity. To characterize the composition of these T cells involved with the pathogenesis of chronic hepatitis B (CHB), we have studied the clonality of VβT cell receptor (TCR)-bearing populations in liver tissue by size spectratyping the complementarity-determining region (CDR3) lengths of TCR transcripts. We have also compared the CDR3 profiles of the lymphocytes infiltrating the liver with those circulating in the blood to see whether identical clonotypes may be detected that would indicate a virus-induced expansion in both compartments. Our studies show that in most of the patients examined, the T cell composition of liver infiltrating lymphocytes is highly restricted, with evidence of clonotypic expansions in 4 to 9 TCR Vβ subfamilies. In contrast, the blood compartment contains an average of 1 to 3 expansions. This pattern is seen irrespective of the patient's viral load or degree of liver pathology. Although the TCR repertoire profiles between the 2 compartments are generally distinct, there is evidence of some T cell subsets being equally distributed between the blood and the liver. Finally, we provide evidence for a putative public binding motif within the CDR3 region with the sequence G-X-S, which may be involved with hepatitis B virus recognition. (HEPATOLOGY 2001;33:1288-1298.) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text