Translocação de bactérias marcadas com 99mTc após isquemia e reperfusão intestinal
| Autor: | Suelene Suassuna Silvestre de Alencar, Aldo Cunha Medeiros, Valbert Nascimento Cardoso, Carlos Teixeira Brandt, Simone Otília Fernandes Diniz, Samir Assi João |
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| Rok vydání: | 2004 |
| Předmět: |
Pathology
medicine.medical_specialty Ischemia 99mTecnécio. Bactéria marcada Spleen Chromosomal translocation medicine.artery medicine Mesenteric lymph nodes Superior mesenteric artery Colony-forming unit business.industry Translocação bacteriana Intestino medicine.disease Small intestine Intestine Isquemia medicine.anatomical_structure Bacterial translocation 99mTechnecium labelled bacteria Mesenteric ischemia Reperfusion Reperfusão Surgery business |
| Zdroj: | Acta Cirúrgica Brasileira v.19 n.4 2004 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC Acta Cirurgica Brasileira, Volume: 19, Issue: 4, Pages: 328-333, Published: AUG 2004 Repositório Institucional da UFRN Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
| ISSN: | 0102-8650 |
| DOI: | 10.1590/s0102-86502004000400003 |
| Popis: | PURPOSE: Ischemia and reperfusion of the small intestine disrupts gut barrier, causes bacterial translocation and activates inflammatory responses. An experimental study was planned to evaluate if 99mTc labelled Escherichia coli translocates to mesenteric lymph nodes, liver, spleen, lung and serum of rats submitted to mesenteric ischemia/reperfusion. Additionally, it was observed if the time of reperfusion influences the level of translocation. METHODS: Forty male Wistar rats underwent 45 minutes of gut ischemia by occlusion of the superior mesenteric artery. The translocation of labelled bacteria to different organs and portal serum was determined in rats reperfused for 30 minutes, 24 hours, sham(S) and controls(C), using radioactivity count and colony forming units/g (CFU). RESULTS: All the organs from rats observed for 24 hours after reperfusion had higher levels of radioactivity and positive cultures (CFU) than did the organs of rats reperfused for 30 minutes, C and S, except in the spleen (p |
| Databáze: | OpenAIRE |
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