Evolution of SIV envelope in morphine-dependent rhesus macaques with rapid disease progression
Autor: | Anil Kumar, Grissell Tirado |
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Rok vydání: | 2006 |
Předmět: |
Sequence analysis
viruses Immunology Molecular Sequence Data Simian Acquired Immunodeficiency Syndrome Biology medicine.disease_cause Virus Evolution Molecular Phylogenetics Virology medicine Animals Vector (molecular biology) Amino Acid Sequence Phylogeny Phylogenetic tree Morphine Simian immunodeficiency virus biology.organism_classification Macaca mulatta Infectious Diseases Viral evolution Lentivirus Disease Progression Simian Immunodeficiency Virus Morphine Dependence |
Zdroj: | AIDS research and human retroviruses. 22(1) |
ISSN: | 0889-2229 |
Popis: | Three morphine-dependent rhesus macaques that developed accelerated AIDS after virus inoculation, along with three control macaques, were followed for evolution of the SIV/17E-Fr envelope. Viral RNA was isolated from plasma samples collected at weeks 6, 12, and 20 postinfection. A 482-nucleotide fragment in the viral env was amplified and cloned into a pCR2.1-TOPO vector. Between 5 and 10 clones were sequenced at each time point from individual monkeys. The sequence analysis showed more mutations in the control animals compared to those seen in the morphine-dependent animals. The virus at different points did not separate completely in phylogenetic analysis. However, the phylogenetic clustering was more apparent in the control animals. Viral diversity and divergence were significantly higher in the control animals. The control animals lost N-glycosylation sites more rapidly. These results suggest that morphine dependence diminished virus evolution in SHIV/SIV-infected rhesus macaques and there was an inverse correlation between virus evolution and onset of clinical disease. |
Databáze: | OpenAIRE |
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