Pathogenesis and Persistence of Increased Epithelial Mucosubstances in the Nasal Airways of Rats and Mice Episodically Exposed to Ethylene
| Autor: | Jon A. Hotchkiss, Ryan A Lewandowski, Daven N. Jackson-Humbles, Shannon M. Krieger, Amy Freeland, Elyse A Eldridge, Jack R. Harkema, James G. Wagner |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Toxicology Pathology and Forensic Medicine Muscle hypertrophy Pathogenesis 03 medical and health sciences Mice 0302 clinical medicine Immune system Internal medicine medicine Animals Lymphocytes Respiratory system Molecular Biology 030304 developmental biology Rhinitis 0303 health sciences Inhalation Exposure business.industry Innate lymphoid cell Cell Biology respiratory system Hyperplasia Ethylenes medicine.disease Immunity Innate Rats Inbred F344 Rats Endocrinology 030228 respiratory system Respiratory epithelium Histopathology business |
| Zdroj: | Toxicologic pathology. 48(7) |
| ISSN: | 1533-1601 |
| Popis: | Rats repeatedly exposed to high airborne concentrations of ethylene develop eosinophilic rhinitis and mucous cell hyperplasia/hypertrophy (MCH) in nasal respiratory epithelium. Mechanisms underlying these lesions are not well understood to inform occupational exposure guidelines. In this study, we determined (1) the nasal histopathology in rats episodically exposed to ethylene, (2) the ethylene-induced nasal histopathology in similarly exposed mice, and (3) how innate lymphoid cells (ILCs) play a role in ethylene-induced MCH. Animals were exposed to 0 or 10,000 ppm ethylene, 6 h/d, 5 d/wk, for 2 weeks and sacrificed 1 day or 2 weeks postexposure. Others received three 2-week exposure blocks separated by 2-week intervals of no exposure. Episodic exposure was chosen to aid in distinguishing irritant from immune responses. Mucous cell hyperplasia/hypertrophy was induced by ethylene in both species. Rats developed a mild, but transient, eosinophilic rhinitis. Mucous cell hyperplasia/hypertrophy was transient in mice, but persistent in rats. Increases in epithelial mucosubstances after 2 weeks of exposure were only present in ILC-sufficient mice, but not in ILC-deficient mice suggesting that ILCs play a role in MCH and overexpression of genes associated with mucus production/secretion. These findings in animals suggest that inhaled ethylene does not act as a sensitizing agent and will not induce allergen-like nasal airway disease. |
| Databáze: | OpenAIRE |
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