Differential N-Linked Glycosylation of Human Immunodeficiency Virus and Ebola Virus Envelope Glycoproteins Modulates Interactions with DC-SIGN and DC-SIGNR
| Autor: | Graham Simmons, George Lin, Frédéric Baribaud, Drew Weissman, Stefan Pöhlmann, Paul Bates, Beth S. Haggarty, George J. Leslie, James A. Hoxie, Houping Ni, Robert W. Doms |
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| Rok vydání: | 2003 |
| Předmět: |
Glycosylation
viruses Immunology Receptors Cell Surface Viral transformation medicine.disease_cause Microbiology Virus Cell Line VP40 Viral Envelope Proteins Viral envelope Polysaccharides Virology Murine leukemia virus medicine Humans Lectins C-Type Ebola virus biology HIV Simian immunodeficiency virus Ebolavirus biology.organism_classification Virus-Cell Interactions DC-SIGN Insect Science biology.protein Cell Adhesion Molecules |
| Zdroj: | Journal of Virology. 77:1337-1346 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.77.2.1337-1346.2003 |
| Popis: | The C-type lectins DC-SIGN and DC-SIGNR [collectively referred to as DC-SIGN(R)] bind and transmit human immunodeficiency virus (HIV) and simian immunodeficiency virus to T cells via the viral envelope glycoprotein (Env). Other viruses containing heavily glycosylated glycoproteins (GPs) fail to interact with DC-SIGN(R), suggesting some degree of specificity in this interaction. We show here that DC-SIGN(R) selectively interact with HIV Env and Ebola virus GPs containing more high-mannose than complex carbohydrate structures. Modulation of N -glycans on Env or GP through production of viruses in different primary cells or in the presence of the mannosidase I inhibitor deoxymannojirimycin dramatically affected DC-SIGN(R) infectivity enhancement. Further, murine leukemia virus, which typically does not interact efficiently with DC-SIGN(R), could do so when produced in the presence of deoxymannojirimycin. We predict that other viruses containing GPs with a large proportion of high-mannose N -glycans will efficiently interact with DC-SIGN(R), whereas those with solely complex N -glycans will not. Thus, the virus-producing cell type is an important factor in dictating both N -glycan status and virus interactions with DC-SIGN(R), which may impact virus tropism and transmissibility in vivo. |
| Databáze: | OpenAIRE |
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