The Adenoids but Not the Palatine Tonsils Serve as a Reservoir for Bacteria Associated with Secretory Otitis Media in Small Children
| Autor: | Anders Jensen, Helena Fagö-Olsen, Christian Hjort Sørensen, Laura Marie Dines |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
adenotonsillectomy Physiology medicine.medical_treatment lcsh:QR1-502 microbiome medicine.disease_cause Biochemistry Microbiology lcsh:Microbiology Host-Microbe Biology Haemophilus influenzae Moraxella catarrhalis 03 medical and health sciences 0302 clinical medicine stomatognathic system Adenoidectomy tonsils Streptococcus pneumoniae otorhinolaryngologic diseases Genetics medicine otitis media with effusion 030223 otorhinolaryngology Molecular Biology Ecology Evolution Behavior and Systematics biology Respiratory tract infections business.industry respiratory system biology.organism_classification QR1-502 Computer Science Applications Lingual tonsils stomatognathic diseases 16S rRNA genes 030104 developmental biology medicine.anatomical_structure Otitis adenoids Modeling and Simulation Tonsil Immunology medicine.symptom business Research Article |
| Zdroj: | mSystems mSystems, Vol 4, Iss 1 (2019) Fagö-Olsen, H, Dines, L M, Hjort-Sørensen, C & Jensen, A 2019, ' The Adenoids but Not the Palatine Tonsils Serve as a Reservoir for Bacteria Associated with Secretory Otitis Media in Small Children ', mSystems, vol. 4, no. 1, e00169-18, pp. 1-17 . https://doi.org/10.1128/mSystems.00169-18 mSystems, Vol 4, Iss 1, p e00169-18 (2019) |
| ISSN: | 2379-5077 |
| DOI: | 10.1128/msystems.00169-18 |
| Popis: | Our findings that the microbiome differs between crypts of the adenoids and crypts of the palatine tonsils, including the relative abundances of potential pathogens such as Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis, may be the stepping stone for further investigation of individual microbiomes in a longitudinal design that includes recording of the fluctuating health status of the child. Such studies may have the potential to lead to new preventive measurements such as implantation of protective nonpathogens at the nasopharynx as an alternative to adenoidectomy. Acute otitis media (AOM), secretory otitis media (SOM), and acute pharyngotonsillitis are the most frequent reasons for visits to general practitioners, pediatricians, and otolaryngologists. Microbial colonization of the epithelial lining of Waldeyer’s lymphatic tissues, consisting of the palatine tonsils, lingual tonsils, adenoids, and Eustachian tube tonsil, is a well-known clinical challenge during infancy due to frequent episodes of upper respiratory tract infections. However, no previous studies have investigated the combined role of the palatine tonsils and the adenoids as a reservoir for pathogens associated with SOM in small children. We analyzed the combined crypt microbiome of the palatine tonsils and adenoids from 14 small children with hyperplasia of the tonsils or adenoids and 14 small children with SOM using 16S rRNA gene pyrosequencing. Our study demonstrated a significant difference between the microbiome of the adenoids and that of the palatine tonsils in the two groups but not between the two anatomical locations within the two groups. In particular, the potential pathogens Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis were almost exclusively found in the adenoids of both patient groups, indicating that the adenoids and not the palatine tonsils are the main reservoir for potential pathogens leading to AOM and SOM. IMPORTANCE Our findings that the microbiome differs between crypts of the adenoids and crypts of the palatine tonsils, including the relative abundances of potential pathogens such as Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis, may be the stepping stone for further investigation of individual microbiomes in a longitudinal design that includes recording of the fluctuating health status of the child. Such studies may have the potential to lead to new preventive measurements such as implantation of protective nonpathogens at the nasopharynx as an alternative to adenoidectomy. |
| Databáze: | OpenAIRE |
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