Modulation effect of Lactobacillus acidophilus KLDS 1.0738 on gut microbiota and TLR4 expression in β-lactoglobulin-induced allergic mice model
| Autor: | Q.-m. Zhang, C. Li, A.-l. Li, P. Du, W.-w. Ni, S.-s. Yu, Zhong Chen, X. Zhang, H.-y. Wu, Y. Li |
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| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Firmicutes Immunology Lactoglobulins Gut flora medicine.disease_cause Microbiology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactobacillus acidophilus medicine Animals Immunology and Allergy Receptor Mice Inbred BALB C biology business.industry Probiotics Lachnospiraceae General Medicine biology.organism_classification Gastrointestinal Microbiome Toll-Like Receptor 4 Disease Models Animal 030228 respiratory system chemistry Allergic response TLR4 Female Milk Hypersensitivity business Histamine 030215 immunology |
| Zdroj: | Allergologia et Immunopathologia. 48:149-157 |
| ISSN: | 0301-0546 |
| DOI: | 10.1016/j.aller.2019.06.002 |
| Popis: | Objectives β-lactoglobulin (β-Lg)-sensitized mice model was employed to investigate the correlation between Lactobacillus acidophilus KLDS 1.0738 (Lap KLDS 1.0738) modulating gut microbiota and inducting Toll-like receptors (TLRs) expression. Methods The alterations of mice fecal microbiota were analyzed by 16S rRNA gene sequencing. The serum cytokines production and TLR4/NF-κB mRNA expression in the colon tissues were measured by ELISA kit and quantitative RT-PCR, respectively. Results The results showed that Lap KLDS 1.0738 pretreatment attenuated β-Lg-induced hypersensitivity, accompanied with a diminished expression of TLR4/NF-κB signaling. Moreover, oral administration of Lap KLDS 1.0738 improved the richness and diversity of fecal microbiota, which was characterized by fewer Proteobacteria phylum and Helicobacteraceae family, and higher Firmicutes phylum and Lachnospiraceae family than allergic group. Notably, TLR4/NF-κB expression was positively correlated with the family of Helicobacteraceae in allergic group, but negatively correlated with the family of Lachnospiraceae, Ruminococcaceae and anti-inflammatory cytokines level. A significant positive correlation was observed between TLR4/NF-κB expression and the production of histamine, total IgE and pro-inflammatory cytokines. Conclusions Intake of Lap KLDS 1.0738 can influence the gut bacterial composition, which might result in recognizing TLRs signaling so as to inhibit allergic response. |
| Databáze: | OpenAIRE |
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