Colloidal Gels with Tunable Mechanomorphology Regulate Endothelial Morphogenesis
| Autor: | Meng-Hsuan Lin, Yuan Yuan, Sukanya Basu, Arati N. Kumar, Smruti K. Nair, Paul J. Cullen, Ballari Sen, Debanjan Sarkar |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
food.ingredient Morphology (linguistics) Capillary action lcsh:Medicine Cell Communication Matrix (biology) Gelatin Article 03 medical and health sciences Colloid 0302 clinical medicine food Human Umbilical Vein Endothelial Cells Humans Colloids lcsh:Science Multidisciplinary Chemistry lcsh:R Endothelial Cells Biomaterial Microstructure Endothelial stem cell 030104 developmental biology Microscopy Electron Scanning Biophysics lcsh:Q Gels 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Endothelial morphogenesis into capillary networks is dependent on the matrix morphology and mechanical properties. In current 3D gels, these two matrix features are interdependent and their distinct roles in endothelial organization are not known. Thus, it is important to decouple these parameters in the matrix design. Colloidal gels can be engineered to regulate the microstructural morphology and mechanics in an independent manner because colloidal gels are formed by the aggregation of particles into a self-similar 3D network. In this work, gelatin based colloidal gels with distinct mechanomorphology were developed by engineering the electrostatic interaction mediated aggregation of particles. By altering the mode of aggregation, colloidal gels showed either compact dense microstructure or tenuous strand-like networks, and the matrix stiffness was controlled independently by varying the particle fraction. Endothelial Cell (EC) networks were favored in tenuous strand-like microstructure through increased cell-matrix and cell-cell interactions, while compact dense microstructure inhibited the networks. For a given microstructure, as the gel stiffness was increased, the extent of EC network was reduced. This result demonstrates that 3D matrix morphology and mechanics provide distinct signals in a bidirectional manner during EC network formation. Colloidal gels can be used to interrogate the angiogenic responses of ECs and can be developed as a biomaterial for vascularization. |
| Databáze: | OpenAIRE |
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