Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth Manduca sexta
Autor: | Kanost, Michael R., Arrese, Estela L., Cao, Xiaolong, Chen, Yun-ru, Chellapilla, Sanjay, Goldsmith, Marian R., Grosse-wilde, Ewald, Heckel, David G., Herndon, Nicolae, Jiang, Haobo, Papanicolaou, Alexie, Qu, Jiaxin, Soulages, Jose L., Vogel, Heiko, Walters, James, Waterhouse, Robert M., Ahn, Seung-joon, Almeida, Francisca C., An, Chunju, Aqrawi, Peshtewani, Bretschneider, Anne, Bryant, William B., Bucks, Sascha, Chao, Hsu, Chevignon, Germain, Christen, Jayne M., Clarke, David F., Dittmer, Neal T., Ferguson, Laura C. F., Garavelou, Spyridoula, Gordon, Karl H. J., Gunaratna, Ramesh T., Han, Yi, Hauser, Frank, He, Yan, Heidel-fischer, Hanna, Hirsh, Ariana, Hu, Yingxia, Jiang, Hongbo, Kalra, Divya, Klinner, Christian, Konig, Christopher, Kovar, Christie, Kroll, Ashley R., Kuwar, Suyog S., Lee, Sandy L., Lehman, Rudiger, Li, Kai, Li, Zhaofei, Liang, Hanquan, Lovelace, Shanna, Lu, Zhiqiang, Mansfield, Jennifer H., Mcculloch, Kyle J., Mathew, Tittu, Morton, Brian, Muzny, Donna M., Neunemann, David, Ongeri, Fiona, Pauchet, Yannick, Pu, Ling -ling, Pyrousis, Ioannis, Rao, Xiang -jun, Redding, Amanda, Roesel, Charles, Sanchez-gracia, Alejandro, Schaack, Sarah, Shukla, Aditi, Tetreau, Guillaume, Wang, Yang, Xiong, Guang-hua, Traut, Walther, Walsh, Tom K., Worley, Kim C., Wu, Di, Wu, Wenbi, Wu, Yuan-qing, Zhang, Xiufeng, Zou, Zhen, Zucker, Hannah, Briscoe, Adriana D., Burmester, Thorsten, Clem, Rollie J., Feyereisen, Rene, Grimmelikhuijzen, Cornelis J. P., Hamodrakas, Stavros J., Hansson, Bill S., Huguet, Elisabeth, Jermiin, Lars S., Lan, Que, Lehman, Herman K., Lorenzen, Marce, Merzendorfer, Hans, Michalopoulos, Ioannis, Morton, David B., Muthukrishnan, Subbaratnam, Oakeshott, John G., Palmer, Will, Park, Yoonseong, Passarelli, A. Lorena, Rozas, Julio, Schwartz, Lawrence M., Smith, Wendy, Southgate, Agnes, Vilcinskas, Andreas, Vogt, Richard, Wang, Ping, Werren, John, Yu, Xiao-qiang, Zhou, Jing-jiang, Brown, Susan J., Scherer, Steven E., Richards, Stephen, Blissard, Gary W. |
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Přispěvatelé: | Publica, Waterhouse, Robert, Universitat de Barcelona |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Genome Insect Gene Expression Moth Computational biology Biology Synteny Biochemistry Genome Article 03 medical and health sciences 0302 clinical medicine Manduca Botany Animals Gene family ddc:576.5 Genomes Molecular Biology Gene Genome size Innate immunity Whole genome sequencing Tobacco hornworm Genètica animal Gene Expression Profiling fungi Pupa Sequence Analysis DNA biology.organism_classification Insect biochemistry Lepidoptera Gene expression profiling 030104 developmental biology Manduca sexta Larva Insect Science Animal genetics Lepidòpters Insect 030217 neurology & neurosurgery |
Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Insect biochemistry and molecular biology Insect Biochemistry And Molecular Biology (0965-1748) (Pergamon-elsevier Science Ltd), 2016-09, Vol. 76, P. 118-147 Insect Biochemistry and Molecular Biology, Vol. 76 (2016) pp. 118-47 Recercat. Dipósit de la Recerca de Catalunya instname |
ISSN: | 0965-1748 |
Popis: | Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.  |
Databáze: | OpenAIRE |
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