The Vasopressin Receptors Colocalize with Vasopressin in the Magnocellular Neurons of the Rat Supraoptic Nucleus and Are Modulated by Water Balance
| Autor: | Alain Rabié, Gérard Alonso, Hélène Orcel, Françoise Moos, Amandine Hurbin |
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| Přispěvatelé: | Biologie des neurones endocrines (BNE), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2002 |
| Předmět: |
Male
Receptors Vasopressin Vasopressin [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology MESH: Neurons Oxytocin Supraoptic nucleus 0302 clinical medicine Endocrinology MESH: Animals In Situ Hybridization Vasopressin receptor Neurons Arginine vasopressin receptor 1B 0303 health sciences Arginine vasopressin receptor 1A MESH: Indicators and Reagents Water-Electrolyte Balance Immunohistochemistry MESH: Gene Expression Regulation Hypothalamus MESH: Receptors Vasopressin Supraoptic Nucleus MESH: Vasopressins hormones hormone substitutes and hormone antagonists medicine.medical_specialty MESH: Rats Vasopressins Neuropeptide Biology 03 medical and health sciences MESH: In Situ Hybridization Internal medicine MESH: Oxytocin medicine Animals RNA Messenger Rats Wistar MESH: RNA Messenger 030304 developmental biology MESH: Immunohistochemistry MESH: Rats Wistar MESH: Male Rats Gene Expression Regulation nervous system MESH: Supraoptic Nucleus MESH: Water-Electrolyte Balance Magnocellular cell Indicators and Reagents 030217 neurology & neurosurgery |
| Zdroj: | Endocrinology Endocrinology, Endocrine Society, 2002, 143 (2), pp.456-466. ⟨10.1210/endo.143.2.8643⟩ |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.143.2.8643 |
| Popis: | Activity of the magnocellular neurons that synthesize vasopressin in the supraoptic and paraventricular nuclei of the hypothalamus is modulated by local release of the neuropeptide within the nuclei. V1a and V1b vasopressin receptor genes are expressed in these cells. The present study reports the localization of V1a and V1b receptors using multiple labeling immunocytochemistry. Both receptors are mainly located in vasopressinergic magnocellular neurons and colocalized with vasopressin in cytoplasmic vesicles dispersed throughout the cell. Possible functional modifications of the mRNA and protein levels of the V1a receptor, the major isoform, were also investigated by semiquantitative in situ hybridization and immunocytochemistry in rats submitted to reduced or increased water intake. V1a mRNA and receptor levels varied with water balance. V1a mRNA level dropped in rats submitted to high water intake. Conversely, dehydration up-regulated the V1a receptor content. These observations suggest that the pathways that regulate the expression of the genes encoding vasopressin and the V1a receptor are linked, which fits the present findings that the two partners are colocalized in cytoplasmic vesicles. Colocalization might explain how V1 autoreceptors are controlled by cell activity and/or local concentration of vasopressin (released locally by the neurons themselves), allowing fine adjustment of magnocellular neuron activity. |
| Databáze: | OpenAIRE |
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